高效液相色谱法测定蛋白粉中的三聚氰胺  

Determination of Melamine in Protein Powder with HPLC

作　　者：苏爱梅[1] 李锋[1] 

机构地区：[1]南通农业职业技术学院,江苏南通226007

出　　处：《湖北农业科学》2011年第2期378-380,共3页Hubei Agricultural Sciences

基　　金：南通农业职业技术学院"青蓝工程"

摘　　要：蛋白粉经萃取,过PCX柱提纯后,采用Symmetry-C18柱,以庚烷磺酸钠缓冲溶液-甲醇(95∶5,V/V)为流动相,流速1.0 mL/min,测定蛋白粉中三聚氰胺的含量。该方法在1～100.0 mg/L范围内,相对标准偏差小于0.04%,标准曲线呈良好的线性关系(R2=0.999 1),最低检出限为1.0 mg/L,样品加标回收率为98.7%～104.0%,相对标准偏差小于0.4%,该方法能快速有效地测定蛋白粉中三聚氰胺的含量。A HPLC method for the determination of melamine in protein powder was developed.Melamine in sample was extracted and purified by PCX column,then separated in Symmetry-C18 column with a mobile phase consisting of sodium heptanesulfonate and methanol（95：5,V/V）at 1.0 mL/min.The method showed a good linear relationship（r=0.999 1）for melamine of 1～100.0 mg/L with relative standard deviation was less than 0.4%,the detection limit was 1.0 mg/L,the recoveries ranged from 98.7%to 104%with relative standard deviations less than 0.04%.The method was a fast and accurate one for detecting melamine in protein powder.

关 键 词：三聚氰胺 阳离子交换柱 蛋白粉 

分 类 号：R991[医药卫生—毒理学]