丙酮酸高产菌诱变选育及发酵工艺优化的初步研究  

Mutation Breeding and Preliminary Optimization of the Fermentation Process for Pyruvic Acid-Producing Strain

作　　者：胡浩然[1] 石勇[1] 陈雄[1] 

机构地区：[1]湖北工业大学生物工程学院发酵工程省部共建教育部重点实验室,湖北武汉430068

出　　处：《化学与生物工程》2011年第2期59-62,共4页Chemistry & Bioengineering

基　　金：湖北工业大学发酵工程省部共建教育部重点实验室2009年开放基金课题(2009KFJJ07)

摘　　要：以光滑球拟酵母(Torulopsis glabrata)HB20为出发菌,经过UV和DES复合诱变选育出一株丙酮酸高产菌HB304。鉴定其为维生素营养缺陷型,摇瓶发酵产酸量比原菌增加了31.2%;优化培养基后其摇瓶发酵产酸量比原菌增加了41.9%。A high pyruvic acid-producing strain HB304 was bred from Torulopsis glabrata HB20 by UV-DES compound mutation.The strain HB304 appeared to be auxotroph.The yield of pyruvic acid was increased by 31.2% than that of the wild strain in flask culture.After optimization about the medium,the yield of pyruvic acid was increased by 41.9% than that of the wild strain in flask culture.

关 键 词：丙酮酸 复合诱变 育种 优化 

分 类 号：TQ921[轻工技术与工程—发酵工程] Q939[生物学—微生物学]