固定化小麦酯酶生物传感器的制备及应用  

Preparation and application of immobilized wheat esterase biosensors

作　　者：王会友[1] 王涛[1] 王振宇[1,2] 于伟[1] 

机构地区：[1]东北林业大学林学院食品科学专业,黑龙江哈尔滨150040 [2]哈尔滨工业大学食品学院,黑龙江哈尔滨150086

出　　处：《食品工业科技》2011年第3期385-389,共5页Science and Technology of Food Industry

基　　金：东北林业大学研究生科技创新资助项目

摘　　要：将固定化酶膜与精密酸度计的玻璃电极连接制备出了生物传感电极探头,并对两种常见的有机磷农药乐果和敌百虫进行测试,结果:乐果浓度在1×10-8～1×10-3mol/L内与小麦酯酶的抑制率呈线性关系,回归方程为y=13.02x+118.7,相关系数为0.995,最低检出限(LDC)为1.7×10-9mol/L;敌百虫浓度在1×10-8～1×10-3mol/L内与小麦酯酶的抑制率呈线性关系,回归方程为y=13.08x+112.7,相关系数为0.997,LDC为1.1×10-8mol/L。以国家标准方法测得结果为对照,进行实际样品检测,检测方程得出的结果回收率为真实值的87.9%～92.3%,而生物敏感膜重复使用20次以内其酶活保存率为初始酶活的90%左右。因此,此传感器可以应用于现场有机磷农药的实验检测等。The immobilized wheat esterase membrane was fixed at the glass electrode precise acidimeter probe to form a biosensor and detected the two kinds of usual organophosphorus pesticides of dimethoate and metrifonate. Result： in the range of dimethoate concentration 1 ×10 -8～1 ×10 -3mol/L,it had linear relation with wheat esterase’s inhibition rate.Regression equation was y =13.02x ＋118.7,correlation coefficient was 0.995,limit detectability（ LDC） was 1.7 ×10 -9 mol/L.In the range of metrifonate concentration 1 × 10 -8 ～1 × 10 -3 mol/L,it had linear relation with wheat esterase’s inhibition rate.Regression equation was y = 13.08x ＋ 112.7,correlation coefficient was 0.997,limit detectability（ LDC） was 1.1 ×10 -8mol/L.In the actual sample detection,compared to the national standard method of control,we concluded that the recovery rate of using dimethoate detection equation was 87.9%～92.3% of the true value,and the repeated using number of bio-sensitive membrane resulted that in 20 times using the preserved activity was 90% of initial’s. The biosensors had the capacity of rapidity organophosphorus pesticide detection.

关 键 词：固定化 小麦酯酶 生物传感器 有机磷农药 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]