一株耐盐苯酚降解菌的分离、鉴定及特性研究  

Isolation,identification and characteristics of a salt-tolerant strain for phenol degradation

作　　者：曲媛媛[1] 周豪[1] 王平[1] 汲广欣[1] 周集体[1] 

机构地区：[1]大连理工大学工业生态与环境工程教育部重点实验室,辽宁大连116024

出　　处：《大连理工大学学报》2011年第1期25-30,共6页Journal of Dalian University of Technology

基　　金：国家自然科学基金资助项目(50608011)

摘　　要：从污水处理厂活性污泥中筛选到一株高效的耐盐苯酚降解菌,经形态观察及26SrRNA序列分析鉴定为假丝酵母菌Candida sp.,命名为W3.该菌株可以在1%～10%NaCl环境中生长,并可以利用苯酚为唯一碳源和能源.苯酚降解的最优条件为pH=7.0,温度30℃,NaCl质量分数5%.在NaCl质量分数为5%、苯酚浓度为1 500mg/L时,12d苯酚的降解率可达到99.8%.菌株可以在高盐条件下降解苯胺、水杨酸等多种芳香化合物,且对混合酚具有一定的降解能力.对菌株的耐盐机理进行了初步研究,发现细胞内四氢嘧啶和甜菜碱的含量随着盐浓度的增大而增加,表明W3在高盐条件下以四氢嘧啶和甜菜碱作为主要的渗透调节物质.A salt-tolerant phenol degrading yeast strain W3was isolated from the sludge of a sewage plant.It was identified as Candida sp.by morphological and 26SrRNA sequence analysis.The strain was capable of growing in the medium with 1%-10% NaCl and utilizing phenol as sole carbon source and energy.It is found that the optimal conditions for growth and degradation are pH 7.0,temperature 30℃,5% NaCl.Under 5% NaCl,1 500mg/L of phenol is degraded by W3within 12 days and the degrading rate is 99.8%.The strain can also degrade various aromatic compounds,such as aniline,salicylic acid,and phenolic mixtures under high salt conditions.The salt-tolerant mechanism of W3shows that the level of ectoine and betaine inside of cells increases with increasing salinity,which indicats that W3can use ectoine and betaine as the osmoprotectant in hypersaline environment.

关 键 词：苯酚 耐盐酵母菌 四氢嘧啶 甜菜碱 

分 类 号：X172[环境科学与工程—环境科学]