Determination of diastereoisomeric alkaloids in urine by capillary electrophoresis with electrochemiluminescence detection  

作　　者：Yan Ming Liu Long Fei Peng Lin Mei Li Juan Liu 

机构地区：[1]College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China Received 1 July 2010

出　　处：《Chinese Chemical Letters》2011年第2期197-200,共4页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(No.21075106);Henan Innovation Project for University Research Talents(No.2005126);Natural Science Foundation of Henan Province of China (No.092300410122)

摘　　要：A new and simple capillary electrophoresis with electrochemiluminescence detection was developed for the separation and the quantification of a pair of diastereoisomenc alkaloids（ephedrine and pseudoephedrine）.The limits of detection（S/N = 3） were 4.5×10^-8 mol/L for ephedrine and 5.2×10^-8 mol/L for pseudoephedrine,respectively.The RSDs of migration time and peak area were less than 1.3 and 2.5%（n = 5）,respectively.The applicability of the propose method was illustrated in the determination of ephedrine and pseudoephedrine in human urine,ephedrine in nasal drops,and the monitoring of pharmacokinetics for pseudoephedrine.A new and simple capillary electrophoresis with electrochemiluminescence detection was developed for the separation and the quantification of a pair of diastereoisomenc alkaloids（ephedrine and pseudoephedrine）.The limits of detection（S/N = 3） were 4.5×10^-8 mol/L for ephedrine and 5.2×10^-8 mol/L for pseudoephedrine,respectively.The RSDs of migration time and peak area were less than 1.3 and 2.5%（n = 5）,respectively.The applicability of the propose method was illustrated in the determination of ephedrine and pseudoephedrine in human urine,ephedrine in nasal drops,and the monitoring of pharmacokinetics for pseudoephedrine.

关 键 词：CE ELECTROCHEMILUMINESCENCE ALKALOIDS Urine analysis PHARMACOKINETICS 

分 类 号：O614.812[理学—无机化学] O657.8[理学—化学]