茶叶糖蛋白调控树突状细胞对T淋巴细胞增殖和CTL活性的影响  

Regulatory Effect of Tea Glycoprotein on Proliferation of T lymphocyte and Activity of CTL Induced by Dendritic Cells

作　　者：韩澄[1] 聂少平[1] 黄丹菲[1] 陈一晴[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《中国食品学报》2011年第1期7-13,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：教育部长江学者和创新团队发展计划(No.IRT0540);食品科学与技术国家重点实验室目标导向项目(SKLF-MB-200806);食品科学与技术国家重点实验室自由探索项目(SKLF-TS-200813)资助

摘　　要：为探讨茶叶糖蛋白(TGP)对小鼠骨髓来源树突状细胞(DCs)表型及功能的影响,采用细胞因子诱导法,以贴壁法获得贴壁单核细胞,添加重组粒细胞-巨噬细胞集落刺激因子(rmGM-CSF)和重组白细胞介素-4(rmIL-4)进行体外诱导培养,倒置显微镜动态观察细胞形态的变化;采用流式细胞术检测DCs的表面标志CD11c和MHCII类分子表达;采用MTT法检测TGP对DCs刺激OVA未致敏或致敏淋巴细胞增殖的影响;初步探讨TGP对DCs抗小鼠SP2/0骨髓瘤功能的影响。结果表明,经TGP作用48h后,树突状细胞形态更加典型、成熟;与阴性对照相比,TGP显著促进树突状细胞表面CD11c和MHCⅡ的表达;TGP可增强DCs的刺激致敏淋巴细胞增殖的能力,说明DCs诱导免疫应答及抗原提呈功能均增强;与未经抗原负载相比,TGP抗肿瘤机制与促进DCs抗原呈递能力有密切的关系,可提高机体对肿瘤细胞的特异性主动免疫功能,而TGP的干预可促进这一功能的提高。茶叶糖蛋白可以促进树突状细胞表型及功能的成熟。To observe the effects of tea glycoprotein（TGP） on the surface molecules expression level and function of murine bone marrow derived dendritic cells.DCs generated from Balb/c murine bone marrow cells were induced by rmGM-CSF and rmIL-4.TGP was added to cells on day 6 of culture for 48 h.Morphology development of DCs was observed by inverted microscope.The phenotypes of DCs were analyzed by using flow cytometry.Antigen presenting ability to allogeneic naive or syngeneic primed T lymphocytes was examined by the lymphocyte proliferation of mixed lymphocyte reaction （MLR） by using MTT assay.Investigate the effect of TGP on cytotoxicity of specific cytotoxic Tlymphocytes （CTL） of SP2/0 tumor cell induced by DCs.The results showed that TGP treated DCs displayed a more matured morpha,with long protrusions,while untreated-DCs displayed shorter protrusions than stimulated DCs.TGP treated immature DCs showed an enhanced cell-surface expression of MHC Ⅱ on CD11c gated DCs.The result demonstrated that TGP can increase antigen presenting ability of DCs to allogeneically naive or syngeneically primed T lymphocytes.It was a conclusion that TGP can promote the cytotoxicity of specific CTL induced by DCs which pulsed with SP2/0 tumor antigen durning the stage of antigen presentation.The results showed that TGP promoted LDH activities released into culture supernatants.TGP can induce phenotypic and functional maturation of murine dendritic cells.

关 键 词：茶叶糖蛋白 树突状细胞 表型 混合淋巴反应 抗原提呈 细胞毒性丁淋巴细胞 

分 类 号：R392[医药卫生—免疫学]