响应面法优化超声波辅助提取荷叶黄酮工艺研究  

Optimization of Technology for Ultrasonic-assisted Extraction of Total Flavonoids from Lotus leaves by Response Surface Methodology

作　　者：郭辉[1] 罗宇倩[1] 章华伟[1] 钱俊青[1] 裴镜澄[1] 

机构地区：[1]浙江工业大学药学院,杭州310014

出　　处：《中国食品学报》2011年第1期119-125,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省科技厅项目(2007C12019)

摘　　要：以荷叶粉末为原料,采用单因素和响应面试验考察超声提取的乙醇体积分数、液固比、超声时间、超声功率和浸泡水浴温度对荷叶黄酮提取效果的影响。试验结果表明,在60%乙醇,液固比20mL/g,超声功率95W条件下提取15min后,于88℃水浴下浸泡10min,黄酮得率11.52%,较单因素试验获得的最佳得率高4.4%。To obtain the optimum operation conditions for extraction of flavonoids from lotus leaves.The single-factor extraction tests and response surface analysis were employed to determine the optimum technological conditions.Concentration of extraction solvent,solid/liquid ratios,ultrasonic power,time and temperature were investigated.The results showed that optimum extraction conditions were as follows：60% ethanol,the liquid/solid ratios 20 mL/g,ultrasonic power 95 W,processing time 15 min,then immerse 10 min under the temperature of 88 ℃.Under these conditions,the extraction rate of total flavonoids is up to 11.52%,which is higher 4.4% than the highest one in single-factor tests.

关 键 词：荷叶 总黄酮 超声提取 响应面设计 

分 类 号：TQ461[化学工程—制药化工]