进口油菜籽中不同转基因品系的检测与分析  

Identification of imported genetically modified rapeseeds

作　　者：李想[1] 潘良文[1] 李俊毅[2] 吕蓉[1] 张舒亚[1] 刘月明[1] 高琴[1] 

机构地区：[1]上海出入境检验检疫局,上海200135 [2]华东理工大学,上海200237

出　　处：《中国油料作物学报》2011年第1期77-82,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家"863"项目(2006AA10Z442);国家转基因重大专项(2009ZX08012-002B);上海市科委创新平台服务项目(10DZ2294102);上海检验检疫局科研项目(HK003-2010)

摘　　要：对我国27批进口油菜籽进行了转基因成分检测和转基因油菜品系组成情况分析。利用已知转基因油菜RT73、T45、Oxy235、Topas19/2、MS1、RF1、RF2、MS8和RF3的特异性检测体系,对初检为阳性的转基因油菜籽样品进行扩增,扩增基因包括bar、PAT、GOX和NPTII。结果共检出25批转基因油菜籽,检出率为92.6%。所有批次均检出品系RT73和MS8×RF3,表明二者为目前我国进口的主要转基因油菜籽。约40%的批次检出MS1×RF1和MS1×RF2,有少量批次检出T45、Oxy235和Topas19/2,每批次均为多品系混合样品。研究表明,列入农业转基因生物安全生产证书(我国农业部颁发)名单的7个转基因油菜品系均有进口,其中以RT73和MS8×RF3为主。Composition of genetically modified（GM） rapeseed events in Shanghai port was identified among 27 lots of rapeseed samples imported from Canada and Ukraine.Event-specific real-time PCR methods suitable for known GM rapeseeds RT73,T45,Oxy235,Topas19/2,MS1×RF1,MS1×RF2 and MS8×RF3 were employed in this study.Results showed that 25 lots of rapeseeds,accounting for 92.6% of total,were positive by detection of the exogenous gene bar,PAT,GOX and NPTII.GM rapeseeds RT73 and MS8×RF3 were detected in all 25 positive lots,indicating that they were the major imported rapeseed events.MS1×RF1 and MS1×RF2 events could be detected in 40% lots.T45,Oxy235 and Topas19/2 could be detected in a few lots.The results indicated that all the seven GM rapeseed events licensed by the Ministry of Agriculture of People＇s Republic of China existed in imported rapeseed samples in Shanghai port.

关 键 词：进口 品系组成 转基因油菜籽 

分 类 号：S565.402[农业科学—作物学]