21个非CODIS STR基因座的遗传多态性  

Genetic Polymorphisms of 21 Non-CODIS STR Loci

作　　者：邵伟波[1,2] 张素华[1,2] 李莉[2] 

机构地区：[1]苏州大学医学部法医学系,江苏苏州215123 [2]司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063

出　　处：《法医学杂志》2011年第1期36-38,共3页Journal of Forensic Medicine

基　　金：上海市科技攻关课题项目(08251203200);上海市自然科学基金项目(08ZR1419800)

摘　　要：目的调查华东汉族人群21个非CODIS STR基因座的遗传多态性并评价其法医学应用价值。方法用AGCU 21+1 STR试剂盒,对华东地区汉族225个无关个体的21个非CODIS STR基因座进行扩增,用3130遗传分析仪检测扩增产物,GeneMapper ID v3.2软件进行分型,采用PowerStats v12.xls和Cervus 2.0分析软件计算常用法医遗传学参数。结果 21个非CODIS STR基因座的频率分布在本组人群中均符合Hardy-Weinberg平衡。杂合度分布为0.596～0.804,个体识别率为0.764～0.948,二联体非父排除率(PEduo)为0.176～0.492,三联体非父排除率(PEtrio)为0.334～0.663,多态信息含量(PIC)为0.522～0.807。二联体累积非父排除率(CPE)为0.999707,三联体CPE为0.9999994,累积个体识别率(CDP)为0.99999999999999999994。结论 21个非CODIS STR基因座具有良好的遗传多态性,能有效运用于个体识别和三联体亲子鉴定,也可作为二联体亲子鉴定案例的补充工具。Objective To investigate genetic polymorphisms of 21 non-CODIS STR loci in Han population from the east of China and to explore their forensic application value.Methods Twenty-one non-CODIS STR loci,were amplified with AGCU 21＋1 STR kit and DNA samples were obtained from 225 unrelated individuals of the Han population from the east of China.The PCR products were analyzed with 3130 Genetic Analyzer and genotyped with GeneMapper ID v3.2 software.The genetic data were statistically analyzed with PowerStats v12.xls and Cervus 2.0 software.Results The distributions of 21 non-CODIS STR loci satisfied the Hardy-Weinberg equilibration.The heterozygosity（H） distributions were 0.596-0.804,the discrimination power（DP） were 0.764-0.948,the probability of exclusion of duo-testing（PEduo） were 0.176-0.492,the probability of exclusion of trios-testing（PEtrio） were 0.334-0.663,and the polymorphic information content（PIC） were 0.522-0.807.The cumulative probability of exclusion（CPE） of duo-testing was 0.999 707,the CPE of trios-testing was 0.999 999 4,and the cumulated discrimination power（CDP） was 0.999 999 999 999 999 999 94.Conclusion Twenty-one non-CODIS STR loci are highly polymorphic.They can be effectively used in personal identification and paternity testing in trios cases.They can also be used as supplement in the difficult cases of diad paternity testing.

关 键 词：法医遗传学 多态现象 遗传 STR 亲子鉴定 

分 类 号：R394[医药卫生—医学遗传学]