β-catenin基因在乳腺癌中的表达和突变  

Study on 13-catenin gene expression and mutation in breast carcinoma

作　　者：何艳姣[1] 徐妙生[1] 刘朝霞[1] 俞进[1] 李光[1] 

机构地区：[1]首都医科大学附属北京天坛医院病理科,100050

出　　处：《肿瘤研究与临床》2011年第2期97-99,共3页Cancer Research and Clinic

摘　　要：目的探讨β—catenin基因在乳腺癌中的表达和突变情况。方法应用免疫组织化学sP法检测119例乳腺癌、72例乳腺腺病和40例正常乳腺组织中β-catenin的表达，分析其与乳腺癌临床病理特征的关系。应用PCR和基因测序的方法检测44例乳腺癌β-catenin外显子3的突变情况。结果40例正常乳腺组织中β—catenin在细胞膜中强表达。乳腺癌β-catenin异常表达率78．15％（93／119）明显高于乳腺腺病44．44％（32／72），组间比较差异有统计学意义（P〈0．01）。β—catenin异常表达与淋巴结转移相关。44例乳腺癌组织中未检测到β-catenin基因外显子3的突变，β-catenin的异常表达率为77．30％（34／44）。结论β-catenin异常表达可作为评估乳腺癌转移预后、指导治疗的指标。乳腺癌组织中β-catenin异常表达不是由β-catenin基因突变所致，可能存在其他机制，有待于进一步研究。Objective To discuss the expression and mutation of β-catenin gene in breast carcinoma. Methods 119 breast carcinomas, 72 adenosis of breast tissues and 40 normal breast tissues were studied. Using immunohistochemistry studies to detect the expressions of β-catenin, then analyzing their relationship with the clinicopathological features of breast carcinomas. Gene mutation of β-catenin （exon 3） in 44 cases of breast carcinoma were analyzed by polymerase chain reaction （PCR） and then by direct sequencing. Results In 40 cases of normal breast tissues, epithelial cells showed equally strong membranous of β-catenin protein at the cell-cell boundaries. The abnormal rate of β-catenin in breast carcinomas （78.15 %） was much higher than that of adenosis of breast tissues （44.44 %）, the significant correlation was found （P-〈0.01）. The abnormal expression of β-catenin was associated with lymph node metastasis （P =0.031）. Mutation of β-catenin gene was not detected in 44 cases of breast carcinoma, while the abnormal rate of β-catenin in 44 cases of breast carcinoma was 77.30 %. Conclusion β-catenin abnormal expression could be considered as an useful marker for determining metastasis and prognosis of human breast carcinoma and for guiding treatment. β- catenin abnormal expression is not caused by β-catenin gene mutation, maybe by other mechanisms. It needs further study.

关 键 词：乳腺肿瘤 Β-CATENIN 突变 

分 类 号：R737.9[医药卫生—肿瘤]