芦笋18S rDNA序列和分类分析  

Sequence and Classification Analysis of 18S rDNA in Asparagus officinalis L.

作　　者：侯进慧[1] 蔡侃[1] 郑宝刚[1] 曹玉朋[1] 王清泉[1] 

机构地区：[1]徐州工程学院食品(生物)工程学院,江苏徐州221008

出　　处：《食品科学》2011年第5期221-223,共3页Food Science

基　　金：徐州市科技计划项目(XZZD0924);徐州工程学院引进人才科研启动项目;徐州工程学院科研项目(XKY2008110);江苏省大学生实践创新计划项目(2010-971)

摘　　要：通过植物基因组提取方法对芦笋的基因组DNA进行提取,采用PCR方法对其18S rDNA片段进行扩增,再经连接、转化、筛选后,获得长度为1684bp的18S rDNA序列。将序列提交后,获得GenBank数据库基因登录号GU217543。分析芦笋18S rDNA的基因序列,确定芦笋与百合目(Liliales)和天门冬目(Asparagales)的多种植物在分类上有很近的关系,从而对分子水平上芦笋的分类有了进一步的了解。In this study,the genomic DNA of Asparagus officinalis L.was extracted by plant genome extraction method,and its fragment of 18S rDNA was amplified by PCR method,followed by ligation,transformation,and screening.An 18S rDNA fragment of 1684 bp in length was sequenced.The accession number of the sequence in Genbank was GU217543.The gene sequence of Asparagus officinalis L.was analyzed.The results indicated that Asparagus officinalis L.had close genetic relationship with the species of Liliales and Asparagales.Based on these investigations,the classification of Asparagus officinalis L.has been further understood at the molecular level.

关 键 词：芦笋 18S RDNA 分类 

分 类 号：S590.23[农业科学—作物学]