Gadolinium induced apoptosis of human embryo liver L02 cell line by ROS-mediated AIF pathway  

作　　者：叶丽华 施喆 刘会雪 杨晓达 王夔 

机构地区：[1]State Key Laboratories of Natural and Biomimetic Drugs at Peking University [2]Department of Chemical Biology,School of Pharmaceutical Sciences,Peking University Health Science Center

出　　处：《Journal of Rare Earths》2011年第2期178-184,共7页稀土学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (20637010, 20671008 and 20971008)

摘　　要：Gd3＋ complexes have a variety of medical applications.In order to shed light on the mechanism of hepatotoxicity of Gd3＋ compounds,we investigated the effects of GdCl3 on human embryo liver cell strand （L02 cells）.The experimental results showed that long-time exposure to GdCl3 resulted in L02 cell apoptosis.The incubation of L02 cells with GdCl3 first induced increase in cellular reactive oxygen species （ROS） and decrease in mitochondrial inner membrane potential （？ψm）.It later resulted in the activation of poly （ADP-ribose） polymerase （PARP） and the release of mitochondrial apoptosis-inducing factor （AIF）.The activation of caspase 3,however,was not observed.Antioxidants could significantly reduce GdCl3-induced decrease of Δψm,release of AIF,and cell apoptosis.Although GdCl3 caused a significant increase in cell membrane permeability in L02,the change of cell membrane permeability was unlikely to be involved in GdCl3-induced cell apoptosis.Overall,our experimental results suggested that GdCl3 induced apoptosis of human embryo liver L02 cell line by ROS-mediated AIF pathway.Gd3＋ complexes have a variety of medical applications.In order to shed light on the mechanism of hepatotoxicity of Gd3＋ compounds,we investigated the effects of GdCl3 on human embryo liver cell strand （L02 cells）.The experimental results showed that long-time exposure to GdCl3 resulted in L02 cell apoptosis.The incubation of L02 cells with GdCl3 first induced increase in cellular reactive oxygen species （ROS） and decrease in mitochondrial inner membrane potential （？ψm）.It later resulted in the activation of poly （ADP-ribose） polymerase （PARP） and the release of mitochondrial apoptosis-inducing factor （AIF）.The activation of caspase 3,however,was not observed.Antioxidants could significantly reduce GdCl3-induced decrease of Δψm,release of AIF,and cell apoptosis.Although GdCl3 caused a significant increase in cell membrane permeability in L02,the change of cell membrane permeability was unlikely to be involved in GdCl3-induced cell apoptosis.Overall,our experimental results suggested that GdCl3 induced apoptosis of human embryo liver L02 cell line by ROS-mediated AIF pathway.

关 键 词：gadolinium chloride MITOCHONDRIA ROS rare earths 

分 类 号：R363[医药卫生—病理学]