Migration characteristics of neural stem cells for glioma cells in vivo and in vitro  

作　　者：Jianxiong Liu Yi Zhang Haixia Liu Li Qiao Xiaolan Xie 

机构地区：[1]Department of Neurosurgery, Gansu Provincial People's Hospital, Lanzhou 730000, Gansu Province, China

出　　处：《Neural Regeneration Research》2011年第1期12-17,共6页中国神经再生研究（英文版）

摘　　要：This study analyzed migration characteristics of neural stem cells （NSCs） in vitro and in vivo, as well as exogenous gene expression in the brain following transfection of NSCs with an enhanced green fluorescent protein （EGFP） gene expression vector plRES2-EGFP plasmid, in glioma experimental models. NSCs slowly migrated towards glioma cells （C6 cells） in culture, although mouse embryonic fibroblasts 3T3 cells did not migrate. NSCs rapidly migrated to the tumor bed, and to tissues surrounding the tumor and metastatic lesions. A large number of EGFP-expressing cells formed distinct colonies in and surrounding the tumor following NSCs-EGFP transfection. EGFP-positive cells aggregated in incubation sites following 3T3-EGFP treatment. Results suggested that NSCs could be used to track tumor cells in vitro. In vivo, transplanted NSCs survived expressed transfected exogenous genes, migrated to the tumor bed, and expressed exogenous genes.This study analyzed migration characteristics of neural stem cells （NSCs） in vitro and in vivo, as well as exogenous gene expression in the brain following transfection of NSCs with an enhanced green fluorescent protein （EGFP） gene expression vector plRES2-EGFP plasmid, in glioma experimental models. NSCs slowly migrated towards glioma cells （C6 cells） in culture, although mouse embryonic fibroblasts 3T3 cells did not migrate. NSCs rapidly migrated to the tumor bed, and to tissues surrounding the tumor and metastatic lesions. A large number of EGFP-expressing cells formed distinct colonies in and surrounding the tumor following NSCs-EGFP transfection. EGFP-positive cells aggregated in incubation sites following 3T3-EGFP treatment. Results suggested that NSCs could be used to track tumor cells in vitro. In vivo, transplanted NSCs survived expressed transfected exogenous genes, migrated to the tumor bed, and expressed exogenous genes.

关 键 词：GLIOMA stem cells gene TRANSFECTION TRACING MIGRATION 

分 类 号：Q421[生物学—神经生物学] Q786[生物学—生理学]