金雀异黄酮对脂多糖处理的软骨细胞增殖抑制的拮抗及抗炎作用  

Antagonistic Action to Inhibition of Lipopolysaccharide-treated Chondrocyte Proliferation and Anti-inflammatory Action of Genistein

作　　者：张城[1] 宋勇[1] 张艺[1] 刘琳[1] 李继斌[1] 

机构地区：[1]重庆医科大学公共卫生学院营养与食品教研室,重庆400016

出　　处：《中国生物制品学杂志》2011年第2期184-186,194,共4页Chinese Journal of Biologicals

摘　　要：目的观察金雀异黄酮(Genistein,GEN)对脂多糖(Lipopolysaccharides,LPS)处理的软骨细胞增殖抑制的拮抗及抗炎作用。方法体外培养新西兰兔关节软骨细胞,将细胞分为正常对照组、LPS单独处理组(10μg/ml)及LPS(10μg/ml)+不同剂量GEN(1、5、10、15μg/ml)处理组,采用MTT法检测各组细胞的增殖活性,RT-PCR法检测各组细胞相关炎性因子IL-1β、诱导型一氧化氮合酶iNOS、转录调节因子p53基因mRNA的表达。结果剂量为1μg/ml的GEN能拮抗软骨细胞经LPS处理后引起的增殖活性降低,且能抑制经LPS处理后软骨细胞IL-1β、iNOS、p53基因mRNA的表达。结论 GEN对LPS处理的软骨细胞可能具有拮抗其增殖抑制及抗炎的作用。Objective To observe the antagonistic action to inhibition of lipopolysaccharide（LPS）-treated chondrocyte proliferation as well as anti-inflammatory action of genistein（GEN）.Methods The chondrocytes of joints of New Zealand rabbits were cultured in vitro,then divided into various groups.The chondrocytes in normal control group were untreated,while those in LPS group were treated with 10 μg/ml LPS,and those in LPS ＋ GEN groups with 10 μg/ml LPS ＋ GEN at dosages of 1,5,10 and 15 μg/ml respectively.The proliferative activities of chondrocytes in various groups were determined by MTT method,and the expressions of mRNAs of infammatory mediators IL-1β,iNOS and p53 by RT-PCR.Results The GEN at a dosage of 1 μg/ml antagonized the decrease of proliferative activity of LPS-treated chondrocytes and inhibited the expressions of mRNAs of infammatory mediators IL-1β,iNOS and p53.Conclusion GEN might has the antagonistic action to inhibition of LPS-treated chondrocyte proliferation and anti-inflammatory action.

关 键 词：金雀异黄酮 软骨细胞 细胞增殖 炎性因子 

分 类 号：R363[医药卫生—病理学] R681.3[医药卫生—基础医学]