高效液相色谱法快速测定大米中的4种烟碱农药残留量  

Determination of Neonicotinoid Residues in Rice by HPLC

作　　者：乙小娟[1] 朱加叶[1] 丁萍[1] 夏拥军[1] 

机构地区：[1]张家港出入境检验检疫局,江苏张家港215600

出　　处：《食品科学》2011年第6期169-172,共4页Food Science

基　　金：江苏省出入境检验检疫局科研项目(2006KJ14)

摘　　要：建立使用高通量(RRHT)色谱柱的高效液相色谱法同时测定大米中呋虫胺、噻虫胺、吡虫啉、吡虫清四种烟碱农药残留量的方法。样品用乙腈提取,采用ENVI-Florisil和Carb复合固相萃取柱萃取净化,液相色谱高通量RRHT色谱柱,258nm紫外测定,外标法定量。检测过程快速准确,2.5min时4种农药可完全分离。样品中加标回收率为75.5%～96.0%,相对标准偏差为0.49%～4.36%,最低检出限达到0.004mg/kg。峰面积与标准溶液质量浓度在0.1～1.0mg/L范围内呈良好的线性关系,线性回归系数大于0.9996。A reverse phase high performance liquid chromatography（RP-HPLC） method has been developed for determining dinotefuran,clothianidin,imidacloprid and acetamiprid residues in rice.Neonicotinoid residues were extracted from the sample by acetonitrile,and then cleaned up with ENVI-Florisil and carb SPE tube.The HPLC detection was performed using a DAD detector in the external standard mode.Results indicated that average recovery rates in spiked samples were in the range of 75.5%-96.0%,the relative standard deviations varied between 0.49% and 4.36% and the detection limit was 0.004 mg/kg.A good linear correlation between peak areas and the concentrations of four neonicotinoid residues was observed in the range of 0.1-1000μg/mL with a calibration coefficient of 0.9996.

关 键 词：呋虫胺 噻虫胺 吡虫啉 吡虫清 液相色谱 高通量色谱柱 固相萃取 大米 

分 类 号：O657.7[理学—分析化学]