mTOR信号通路蛋白在人脑胶质瘤中的表达及其临床意义  

Expression of mTOR signaling pathway proteins in gliomas and its clinical significance

作　　者：李学元[1,2] 贾爱华[3] 任玉波[3] 李鑫[1] 马翔宇[2] 张连群[1] 张学广[1] 李新钢[2] 

机构地区：[1]聊城市人民医院神经外科,山东聊城252000 [2]山东大学齐鲁医院神经外科,山东济南250012 [3]聊城市人民医院病理科,山东聊城252000

出　　处：《中国肿瘤生物治疗杂志》2011年第1期75-79,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目( No. 30672163);山东省自然科学基金资助项目( No. Y2007C108)~~

摘　　要：目的:研究哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)信号通路蛋白在人脑胶质瘤中的表达及其临床意义。方法:选取山东大学齐鲁医院和聊城市人民医院神经外科2007年7月至2010年5月间手术切除并经病理证实的人脑胶质瘤石蜡标本87例(Ⅰ～Ⅱ级27例、Ⅲ级24例、Ⅳ级36例),应用免疫组织化学法检测胶质瘤组织中mTOR信号通路关键蛋白pAKT、pmTOR和p-p70S6K的表达,分析它们与胶质瘤患者临床病理特征的关系。结果:pAKT、pmTOR和p-p70S6K在各级胶质瘤中的阳性率分别为:Ⅰ～Ⅱ级70.4%(19/27)、Ⅲ级70.8%(17/24)、Ⅳ级75.0%(27/36),Ⅰ～Ⅱ级77.8%(21/27)、Ⅲ级75.0%(18/24)、Ⅳ级72.2%(26/36),Ⅰ～Ⅱ级63.0%(17/27)、Ⅲ级70.8%(17/24)、Ⅳ级80.6%(29/36);各阳性表达率间无明显差异(P>0.05),但它们的表达水平均随着胶质瘤病理级别的增高而升高(P=0.0001,0.0063,0.0001),而与患者的性别、年龄、肿瘤大小和术前KPS评分等参数无关。pAKT、pmTOR和p-p70S6K三者共表达于42例胶质瘤组织中,在其余45例中仅有一种或两种表达。结论:mTOR信号通路蛋白在胶质瘤的发生、发展中起着重要的作用,可能是胶质瘤治疗的潜在靶点。Objective：To study the expression of mammalian target of rapamycin （mTOR） signaling pathway protein in glioma patients and to discuss the related clinical significances. Methods：Eighty-seven paraffin samples from glioma patients （27 of Ⅰ-Ⅱ grade,24 of Ⅲ grade,24 of Ⅳ grade）,who were pathologically diagnosed in Department of Neurological Surgery,Qilu Hospital and People’s Hospital of Liaocheng from Jul. 2007 to May. 2010,were obtained. Immunohistochemistry was used to examine the expression levels of 3 key proteins of mTOR signaling pathway,pAKT,pmTOR and p-p70S6K,in the glioma tissues,and their relationship with clinicopathological characteristics of glioma patients was discussed. Results：The positive rates of pAKT,pmTOR and p-p70S6K in different grades of glioma tissues showed no significant difference （P〉0.05）,with pAKT being 70.4% （19/27） inⅠ-Ⅱ grade,70.8% （17/24） in Ⅲ grade,75% （27/36） in Ⅳ grade; pmTOR being 77.8% （21/27） inⅠ-Ⅱ grade,75.0% （18/24） in Ⅲ grade,72.2% （26/36） in Ⅳ grade; and p-p70S6K being 63% （17/27） inⅠ-Ⅱ grade,70.8% （17/24） in Ⅲ grade,80.6% （29/36） in Ⅳ grade tissues. The positive rates increased with the increase of pathological grades （P=0.0001,0.0063,0.0001,respectively）,and they showed no relationship with gender,age,tumor volume,or KPS score of glioma patients. pAKT,pmTOR and p-p70S6K co-expressed in 42 cases; only one or two proteins were co-expressed in another 45 cases. Conclusion：mTOR signaling pathway proteins may play important roles in the development and progression of glioma,and they may serve as potential targets in treatment of glioma.

关 键 词：胶质瘤 MTOR AKT P70S6K 信号转导通路 

分 类 号：R739.41[医药卫生—肿瘤] R730.2[医药卫生—临床医学]