局部高频电刺激海马对海人酸致痫大鼠海马细胞外谷氨酸和γ-氨基丁酸释放的影响  

The effect of high frequency stimulation of epileptic foci on the release of glutamate and gama-aminobutyric acid in hippocampus of the kainic acid-kindled rats

作　　者：林婉挥[1] 黄华品[1] 林明星[1] 陈圣根[1] 

机构地区：[1]福建医科大学附属协和医院神经内科,福州350001

出　　处：《中国应用生理学杂志》2011年第1期88-92,共5页Chinese Journal of Applied Physiology

基　　金：福建省教育厅基金资助项目(JA08097)

摘　　要：目的:通过高频电刺激海人酸癫痫模型大鼠海马,观察海马细胞外谷氨酸(Glu)和γ-氨基丁酸(GABA)的动态变化。方法:将SD大鼠分成4大组(n=10):①空白组;②海人酸组;③假刺激组:植入刺激电极未予电刺激;④电刺激组:海人酸注射后予130 Hz电刺激。利用微透析技术收集不同时段海马细胞外液,应用高效液相-荧光检测法测定收集液Glu、GABA的浓度。结果:注射海人酸后Glu明显升高,并持续至第14天,电刺激使Glu明显下降;而注射海人酸后GABA呈短暂性升高,后逐渐下降于第4天后保持稍高于正常水平,电刺激并无明显改变GABA的水平。结论:海马细胞外Glu下降在海马电刺激治疗癫痫中起到重要作用;高频电刺激海马选择性地减少谷氨酸能神经元活动,但不影响GABA的释放。Objective： To observe the dynamics of hippocampal release of glutamate（Glu） and γ-aminobutyric acid（GABA） in epilepsy（TLE） after administration with high frequency stimulation（HFS）.Methods： The SD rats were divided into four groups（n=10）： ①Control group（KB） the rats were injected intraperitoneally with saline 0.9%.②Kainic acid（KA） group： the rats were injected with KA.③Pseudo-deep brain stimulation（DBS） group： the KA-induced rats were implanted with rheophores alone.④DBS group： KA induced-rats with DBS in hippocampal epileptic foci.We then collected hippocampal extracellular fluid by microdialysis and the levels of Glu and GABA were measured by high-performance liquid chromatography（HPLC） and fluorescence detection.Results： There was no difference in the baseline of Glu and GABA in the four groups.In contrast,a significant increase in the content of Glu and GABA was shown in the three periods of KA-kindled seizures.Electrical stimulation of hippocampus resulted in a decrease of hippocampal Glu contents,while there was no change in GABA contents.Additionally,HFS of hippocampus normalized the Glu/GABA ratio in the chronic period of seizures.Conclusion： The high frequency stimulation of epileptic foci may protect against seizures by modulating the extracellular release of hippocampal Glu.

关 键 词：癫痫 电刺激 海马 微透析 谷氨酸 Γ-氨基丁酸 

分 类 号：R742.1[医药卫生—神经病学与精神病学]