一株煤矿地下水硫酸盐还原细菌的分离、鉴定及性质研究  

Isolation and identification of a SRB strain from coal mine groundwater and its characteristics analysis

作　　者：葛晓光[1] 杨柳[2] 彭申华[2] 李锋[1] 

机构地区：[1]合肥工业大学资源与环境工程学院,安徽合肥230009 [2]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《合肥工业大学学报（自然科学版）》2011年第3期420-423,共4页Journal of Hefei University of Technology：Natural Science

基　　金：安徽省科技攻关计划资助项目(07010202058)

摘　　要：从煤矿井下700m深处含水层的钻孔涌水中分离获得一株硫酸盐还原细菌GY-2，该菌株革兰氏反应阴性，有芽孢，菌体棒状，能运动，氧化酶阴性；通过16S rRNA系统发育学分析，确定菌株GY-2为滴状脱硫肠状菌（Desulfotornaculum guttoideum）。GY-2菌株为兼性厌氧菌，在含有Fe2＋的条件下生长良好；该菌株生长的pH值范围介于6．O～9．0之间，最适pH值为7．O～8．5，生长温度范围为20～45℃，最适温度为30～40℃；该菌株对盐度的耐受力较弱，生长的最适盐度为0．05％～1．O％。A sulfate-reducing bacteria（SRB） strain named GY-2 was isolated from the groundwater in a 700 m deep aquifer at a coal mine. The cells of strain GY-2 were found to be Gram-negative, spore forming, rod-shaped, motile and oxidase-negative. Phylogenetic analysis based on 16S rRNA sequence identified the strain GY-2 as Desulfotomaculum guttoideurn. Strain GY-2 is a faeultative anaerobe and grows well as Fe2＋ is presented. The pH range for strain GY-2 growth is 6.0-9.0 （optimum pH value is 7.0-8.5） and the temperature range is 20-45℃ （optimum temperature is 30-40 ℃）. The salinity tolerance of strain GY-2 is poor and the optimum salinity range is 0. 05%-1. 0%.

关 键 词：硫酸盐还原菌 矿井含水层 16S RRNA 

分 类 号：X172[环境科学与工程—环境科学]