苹果渣固体高密度培养菌体蛋白研究  

Production of single-cell protein from apple pomace by high-density solid-state fermentation

作　　者：张宗舟[1] 赵慧[2] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741000 [2]天水师范学院工学院,甘肃天水741000

出　　处：《中国酿造》2011年第3期105-107,共3页China Brewing

基　　金：2008年国家科技支持计划项目(2007BAD89B17)

摘　　要：利用航空诱变获得的优良菌种—黑曲霉ZM-8和啤酒酵母、热带假丝酵母以及白地霉复配制成复合菌剂,发酵苹果渣,粗蛋白含量显著提高。正交试验复合菌剂的最佳配方为酿酒酵母(S.cerevisiae)0.5g;热带假丝酵母(Candida tropicalis)1.0g;白地霉菌(Geotrichum candidum)1.5g;黑曲霉突变株ZM-8(A.niger ZM-8)1.5g。最适培养基配方为白糖4g,硫铵7g,尿素2g,食盐2g;产物中粗蛋白的含量达28.6%,提高了335%。The crude protein content was significantly increased by mutagenic strains-Aspergillus niger ZM-8, Saccharomyces cerevisiae, Candida tropicalis and Geotrichum candidum using apple pomaee as raw material. The optimal formulation of complex microbial community was obtained by orthogonal experiments as follows： S. cerevisiae 0.5g, Candida tropicalis 1.0g, Geotrichum candidum 1.5g and A. niger ZM-8 1.Sg. The optimal medium was as follows： sugar 4g, ammonium sulfate 7g, urea 2g and salt 2g. Under these conditions, the crude protein content of the product was 28.8%, which was increased by 335% compared to that before optimization.

关 键 词：黑曲霉ZM-8 复合菌剂 单细胞蛋白:苹果渣 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]