荸荠离体培养技术的初步研究  

Study on technique of the culture of Chinese water chestnut in vitro .

作　　者：陈利萍[1] 吕家龙[1] 唐永红 喻景权[1] 汪炳良[1] 

机构地区：[1]浙江大学园艺系,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》1999年第5期524-526,共3页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省教委资助

摘　　要：以荸荠（Ｅｌｅｏｃｈａｒｉｓｔｕｂｅｒｏｓａ Ｒｏｅｍ ． ｅｔ． Ｓｃｈｕｌｔ）品种“大红袍”中心芽的芽尖为外植体，在ＭＳ＋ ６ＢＡ １．０ ｍ ｇ／Ｌ＋ ＮＡＡ ０．１ ｍ ｇ／Ｌ培养基中诱导产生了丛生叶状茎，叶状茎继代培养在 ＭＳ附加不同激素浓度的 ６ＢＡ 和ＣＰＰＵ 培养基中，当６ＢＡ，ＣＰＰＵ 浓度分别为３ ｍ ｇ／Ｌ，０．３ ｍ ｇ／Ｌ时，增殖系数达到最高，为４．００ 左右．叶状茎生根后移栽到大田中，可同以荸荠球茎为繁殖体一样产生球茎．在荸荠收获期测定单荠重和直径，结果表明，试管苗在整齐度上均优于对照。The shoot tips of Chinese water chestnut ( Eleocharis tuberosa Roem. et Schult) cultivars ‘Dahongpao' were used as explants, and clumps of leaf like stems were induced on MS medium containing 1.0 mg/L 6 BA and 0.1 mg/L NAA. These stems subcultured on MS medium supplying different concentrate 6 BA or CPPU. When the concentrate of 6 BA was 3 mg/L, and CPPU was 0.3 mg/L, the propagation coefficient reached the greatest. which was about 4.00. After rooting, the plantlets were transferred to the fields, and yielded corms as the corms breeding. The single corm weight and diameter were measured in the harvest time. The results showed that the plantlets surpassed the control, and the difference in single corm weight was very remarkable.

关 键 词：荸荠 组织培养 繁殖 

分 类 号：S645.30[农业科学—蔬菜学]