硫代巴比妥酸光度法测定香草醛  

The Study and Application of Determination of Vanillin by Spectrophotometry with Thiobarbituric Acid

作　　者：何新亚[1] 刘金霞[1] 曹晓红 叶英植 

机构地区：[1]郑州大学化学化工学院,郑州450052

出　　处：《分析试验室》1999年第5期56-58,共3页Chinese Journal of Analysis Laboratory

摘　　要：香草醛在强酸性介质中与硫代巴比妥酸（ＴＢＡ） 反应生成黄绿色的化合物，建立了可见光分光光度法测定香草醛方法。线性范围１×１０－ ６～１５×１０－ ４ｍ ｏｌ／Ｌ，检出限为４４７×１０－ ８ｍ ｏｌ／Ｌ。对冰淇淋、麦片等进行了其中香草醛含量测定。A spectrophotometric determination of vanillin based on the reaction between vanillin and thiobarbituric acid in hydrochloric acid solution is described.The absorbance is measured at 438nm.The linear range of the calibration curve is 1×10 -6 ～1 5×10 -4 mol/L.The detection limit of vanillin is 4.47×10 -8 mol/L.This method for determination of vanillin was proved to be simple,rapid and accurate.It is used for the determination of vanillin in some foods successfully.

关 键 词：测定 香草醛 硫代巴比妥酸 分光光度法 

分 类 号：TS202[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程]