乙型肝炎病毒A1762T/G1764A突变与原发性肝细胞癌的关系  

Associations between A1762T/G1764A mutations of hepatitis B virus and the risk of hepatocellular carcinoma

作　　者：倪秀莹[1,2] 王涛 杨伟 鞠玲燕 蒋泽辉 

机构地区：[1]解放军潍坊89医院传染科,山东潍坊261021 [2]潍坊医学院,山东潍坊261042 [3]解放军潍坊89医院病理科,山东潍坊261021

出　　处：《中国现代普通外科进展》2011年第1期59-61,74,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:对原发性肝细胞癌(HCC)、慢性乙型病毒性肝炎(CHB)及肝硬化(LV)患者肝组织中乙型肝炎病毒(hepatitis B virus,HBV)X基因基本核心启动子区(BCP)常见变异位点A1762T/G1764A进行检测分析,以探讨HBVX基因A1762T/G1764A变异与HCC发生发展的关系。方法:采用聚合酶链反应(PCR)方法检测67例患者(包括37例HCC,35例非HCC患者)肝组织中HBV X基因,并对PCR产物进行基因测序分析。结果:HCC组患者A1762T/G1764A突变率为85.7%(30/35),非HCC组患者(包括CHB及LV)A1762T/G1764A突变率为40.6%(13/32),HCC组双突变率明显高于非HCC组(P<0.001)。结论:HBVA1762T/G1764A突变在HCC发生发展中可能起重要作用。Objective： To explore the association of the A1762T/G1764A mutant with hepatocelluar carcinogenesis by detecting the frequent mutation sites in basal core promoter（BCP） of HBV X gene in the liver tissues of the patients with hepatocellular carcinoma（HCC）、chronic hepatitis B（CHB） and liver cirrhosis（LV）.Methods： Detecting the HBV X gene in 67 patients including 37 patients with HCC,35 patients with CHB and LV using polymerase chain reaction（PCR）,then the obtained PCR products were sequenced.Results： In the HCC,the rate of HBV A1762T/G1764A mutation was 85.71%（30/35）;in the non-HCC（including the patients with CHB and LV）,the rate of mutation was 40.63%（13/32）.Obviously,the A1762T/G1764A double mutant rate in the HCC was higher than the non-HCC,the difference was significant（P0.001）.Conclusion： HBV A1762T/G1764A mutation may be important in hepatocarcinogenesis.

关 键 词：肝细胞癌 HBVX基因 突变 

分 类 号：R735.7[医药卫生—肿瘤]