酒精-沼气双发酵耦联工艺中SO_4^(2-)的控制  

SO_4^(2-) control in ethanol-methane coupled process

作　　者：王欣[1] 张成明[1] 姜立[1] 陈程[1] 张建华[1] 毛忠贵[1] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122

出　　处：《生物加工过程》2011年第2期33-38,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家高技术研究发展计划(863计划)资助项目(2008AA10Z338)

摘　　要：SO42-是"酒精-沼气双发酵耦联工艺"稳定运行的重要抑制物。在耦联工艺中,以中温厌氧出水代替自来水配料进行酒精发酵。通过无预糖化工序的"同步糖化发酵"技术研究,将酒精发酵的初始pH提高到6.0,H2SO4的消耗降低了50%,使SO42-的质量浓度维持在3 g/L的沼气发酵安全范围内。在进行高浓度酒精发酵时,糖化酶的添加量为每克木薯添加140 U,发酵54 h,最终酒精体积分数达14.7%。糖化酶添加量与常规酒精发酵用量相比,每克木薯增加了糖化酶20 U。SO42- is an important inhibitory substance for the stable operation of the ethanol-methane coupled process.In the coupled process,the mesothermal anaerobic effluent,instead of tap water was used for medium preparation of the ethanol fermentation.The dosage of sulfate reduced 50% through elevating the initial medium pH to 6.0 in the simultaneous saccharification and fermentation without pre-saccharification,resulting in the concentration of sulfate at 3 g/L,it was safe to the methane fermentation.In the very high concentration ethanol fermatation,the dosage of glucoamylase was 140 U/g cassava,fermentation time was 54 h,and the final ethanol concentration was 14.7%（v/v）.In the optimized process,the glucoamylase dosage increased 20 U/g cassava compared with that in the traditional process.

关 键 词：木薯 燃料乙醇 同步糖化发酵 硫酸 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]