高效液相色谱-荧光法检测富含淀粉和糖类药材中黄曲霉毒素的研究  

Determination of Aflatoxin in Traditional Chinese Herbal Medicine Rich in Starch or Sugar by HPLC

作　　者：傅武胜[1,2,3] 刘晓霞[1,4] 邱文倩[1] 吕华东[1] 郑奎城[1] 里南[5] 

机构地区：[1]福建省疾病预防控制中心,福州350001 [2]福建医科大学 [3]福建省疾病预防控制中心教学基地,福州350001 [4]柘荣县食品药品监督管理局,福建352100 [5]福建农林大学食品科技学院,福州350002

出　　处：《海峡预防医学杂志》2010年第6期1-4,共4页Strait Journal of Preventive Medicine

基　　金：福建省科技计划重点项目(No.2008Y0029)

摘　　要：[目的]采用免疫亲和净化手段和液相色谱检测技术,建立富含淀粉、糖类药材中黄曲霉毒素(AF)的测定方法,为福建地产中药材AF的检测和污染调查提供技术支撑。[方法]样品粉末经80%甲醇高速均质提取,用免疫亲和柱(IAC)净化,用HPLC测定,C18反相色谱柱分离,荧光检测器(FLD)测定。[结果]4种AF的最低检出浓度0.02～0.10μg/kg,定量限0.08～0.4μg/kg;以太子参等为加标基质,加标水平为6～24μg/kg(AF总量),AF平均回收率68.7%～113.0%,RSD为1.4%～13.7%。[结论]富含淀粉、糖类药材样品AF含量可用HPLC-FLD测定,准确度高、精密度好,满足欧盟对食品饲料中AF检测的要求。[Objective] To develop a method for quantifying aflatoxins（AF） in traditional Chinese herbal medicine（TCM） rich in starch or sugar and provide technical support for AF detection and contamination control.[Methods] The powdered sample was extracted with 80% methanol.The extract solution was cleaned up by commercial immunoaffinity column（IAC） containing antibody specific to AF.The derivatized eluent was separated by HPLC with C18 column and detected by fluorescence detector（FLD）.[Results] The limit of detection and limit of quantification were 0.02-0.1 and 0.08-0.4 μg/kg in 4 kinds of AF.AF standard solution was spiked to the AF-undetected matrices,such as pseudostellaria heterophylla and so on,at levels of 6-24 μg/kg.The average recoveries varied from 68.7 % to 113.0% and RSD = 1.4%-13.7%.[Conclusions] AF in TCM rich in starch or sugar can be detected by HPLC-FLD method and the accuracy and precision meet the requirement of European Commission for AF determination of food and feed.

关 键 词：中药材 黄曲霉毒素 免疫亲和净化 高效液相色谱 

分 类 号：R282[医药卫生—中药学] O657.7[医药卫生—中医学]