海参共生微生物HS-3 Alternaria sp.次级代谢产物的研究  

The Secondary Metabolites of the HS-3 Alternaria sp.Fungus Associated with Holothurians

作　　者：刘昌衡[1,2] 夏雪奎[3,2] 齐君 张永刚[3,2] 袁文鹏[1,2] 孟秀梅[1,2] 贾爱荣[1,2] 孙永军 胡炜 

机构地区：[1]山东省科学院生物研究所,山东济南250014 [2]山东省应用微生物重点实验室,山东济南250014 [3]山东省科学院中日友好生物技术研究中心,山东济南250014 [4]中国海洋大学医药学院,山东青岛266003 [5]好当家集团有限公司,山东威海264305

出　　处：《中药材》2010年第12期1875-1877,共3页Journal of Chinese Medicinal Materials

基　　金：国家863项目(2007AA091805);山东省优秀中青年科学奖励基金资助项目(BS2009HZ004);山东省科学院博士基金[(2008)102No25]

摘　　要：目的:对自山东半岛芝罘岛海域的刺参中分离出的HS-3 Alternaria sp.真菌的次级代谢产物进行研究。方法:利用土豆培养基对该菌进行培养,通过薄层层析、柱层析、高效液相等分离手段,从HS-3的培养液中分离出4个化合物。结果:经过现代波谱手段,分别鉴定为:1-羟基-3-甲基-9,10-二酮蒽醌(1)、大黄酚(2)、sterigmatocystin(3)、脑苷酯类化合物(4)。结论:该研究为深入研究开发刺参提供了科学数据,这4个化合物首次从海参的共生微生物中得到。Objective：The metabolites of HS-3 associated with holothurians were studied,which was identified by molecular biology as Alternaria sp..Methods： The holothurians were gathered from the Sea of Zhifu Islet,Shandong Province.HS-3 Alternaria sp.was culternitived in potato medium,and four compound was got by TLC,chromatography and HPLC,and 1-hydroxyl-3-methylanthracene-9,10-dione（1）,chrysophanol（2）,sterigmatocystin（3）and cerebroside（4） were elucated by modern spectrum.Conclusion： All of this provides scientific data for further study of holothurians,and the four coumpouns are isolated from the microbe associated with holothurians for the firt time.

关 键 词：海参 共生微生物 ALTERNARIA sp. 次级代谢产物 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]