谷胱甘肽包被的CdSe/CdS纳米粒子对前列腺癌细胞的荧光标记  

CdSe/CdS Core-shell QDs-based Prostate-specific Antigen Probe for Human Prostate Cancer Cell Imaging

作　　者：董微[1] 葛欣[1] 隋丽丽[1] 尚志航[1] 

机构地区：[1]沈阳医学院基础医学院化学教研室,辽宁沈阳110034

出　　处：《沈阳医学院学报》2011年第1期9-12,18,共5页Journal of Shenyang Medical College

基　　金：沈阳医学院科研青年基金(No.20072018)

摘　　要：目的:以谷胱甘肽(GSH)作为稳定剂,在水溶液中制备稳定的CdSe/CdS核/壳结构的纳米量子点。方法:将CdSe/CdS量子点与前列腺癌特异性抗原(PSA)连接,制备水溶性CdSe/CdS-IgG复合物探针,采用直接和间接两种方法对前列腺癌细胞进行标记成像。结果:用该探针对前列腺癌细胞成像清晰,与传统的异硫氰酸荧光素(FITC)相比,具有更强的荧光强度和光稳定性。结论:间接标记法利用一抗和二抗之间的结合降低了非特异性吸附,量子点探针在细胞表面呈现出明显的荧光信号。Objecttive：To prepare fluorescent CdSe/CdS core-shell quantum dots（QDs） capped highly by L-glutathione with an aqueous approach.Method：The as-prepared QDs were linked to prostate-specific antigen（PSA）and immunoglobulin G（IgG） for the indirect labeling of fixed prostate cancer cells.Results：QDs-based probes were ideal fluorescent labels with excellent spectral properties and photostability and they were much better than organic dyes making.Conclusions：They are very suitable in target detection.Meanwhile,the indirect labeling shows much better specificity than the direct labeling.

关 键 词：CdSe/CdS纳米量子点 免疫标记 前列腺癌细胞 直接标记 间接标记 

分 类 号：R737.25[医药卫生—肿瘤]