OGCP代谢途径及Parkin对其代谢的影响  

Metabolic Pathways of OGCP and the Influence of Parkin Protein on the Metabolism of OGCP

作　　者：王春喻[1,2] 曹立[2,3] 唐北沙[2] 张海南[1,2] 郭纪锋[2] 廖书圣[2] 汤建光[1] 严新翔[2] 谭利明[1] 

机构地区：[1]中南大学湘雅二医院神经内科,长沙410011 [2]中南大学湘雅医院神经内科,长沙410008 [3]上海交通大学附属瑞金医院神经内科,上海200025

出　　处：《四川大学学报（医学版）》2011年第2期157-160,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号81000542;30370515;30570638);国家高技术研究发展863计划(2004AA227040);国家重点基础研究发展973计划(2006cb500700);国家十.五科技公关计划(2004BA720A03);青年教师助推项目(201012200211)

摘　　要：目的 研究α-酮戊二酸载体蛋白（2-oxoglutarate carrier protein,OGCP）代谢途径及Parkin蛋白对OGCP代谢的影响。方法 使用特异的蛋白酶体抑制剂和蛋白酶抑制剂分别抑制蛋白酶体和蛋白酶的活性,研究OGCP代谢途径;采用同位素脉冲追踪实验研究OGCP的更新速率,并研究Parkin蛋白对OGCP代谢的影响。结果 蛋白酶体抑制剂和蛋白酶抑制剂均可以抑制OGCP的降解;OGCP的代谢半衰期约为8～10 h,而过表达Parkin蛋白能够加快OGCP的降解。结论 OGCP采取蛋白酶体途径和溶酶体途径进行降解,且Parkin蛋白能促使OGCP降解。Objective To study the metabolic pathways of 2-oxoglutarate carrier protein（OGCP）and the influence of parkin protein on the metabolism of OGCP.Methods The OGCP metabolic pathways were identified through inhibiting proteasome activities with specific proteasome inhibitors and protease inhibitors.The isotope pulse-chase experiments were performed to measure the turnover rate of OGCP and to study the influence of parkin protein on the metabolism of OGCP.Results Proteasome inhibitors and protease inhibitors inhibited OGCP degradation.The OGCP metabolism had a half-life of about 8-10 h.Overexpression of parkin protein accelerated the OGCP degradation.Conclusion OGCP degrades through proteasome and lysosome degradation pathways.The degradation of parkin protein can promote the degradation of OGCP.

关 键 词：OGCP Parkin蛋白 代谢途径 

分 类 号：R742.5[医药卫生—神经病学与精神病学]