酵母产γ-氨基丁酸发酵培养基的优化  

Optimization of Culture Medium for Gamma-aminobutyricAcid Produced by Saccharomyces Cerevisiae Strain

作　　者：胡超[1] 黄丽华[1] 左斌[1] 谢达平[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《现代生物医学进展》2011年第3期482-484,共3页Progress in Modern Biomedicine

摘　　要：目的:提高酵母产γ-氨基丁酸的能力。方法:采用单因素及正交设计实验对酵母产γ-氨基丁酸(GABA)的培养基进行优化。结果:确定最适碳源为葡萄糖,最佳氮源为蛋白胨和硫酸铵复合氮源,合适的无机盐为KH2PO4;最佳发酵培养基为3%葡萄糖,3%蛋白胨,0.3%(NH4)2SO4和0.1%KH2PO4。在此培养条件下,摇瓶发酵可以获得1.690g.L-1的GABA产量。结论:发酵培养基的优化,提高了菌株产γ-氨基丁酸的能力。Objective：To improve Gamma-aminobutyric acid（GABA） production by Saccharomyces cerevisiae.Methods：The culture medium was researched by single factor and orthogonal experiment.Results：The optimum carbon and nitrogen sources were glucose and the mixture of peptone and（NH4）2SO4,the optimal inorganic salt was KH2PO4.The preferable medium was as follows：3% glucose,3% peptone,0.3%（NH4）2SO4,and 0.1% KH2PO4.Under the optimum conditions,the content of GABA reached 1.690 g.L-1.Conclusion：The production of GABA was increased under the optimal conditions.

关 键 词：Γ-氨基丁酸 酵母 培养基优化 

分 类 号：Q815[生物学—生物工程] TQ920.6[轻工技术与工程—发酵工程]