生黑醋酸杆菌的太空诱变选育  

Mutation of Gluconbacter melanogenus in a Two-step Fermentation for Vitamin C Production by Space Flight

作　　者：荆士华[1,2] 安海英 李春杰 李野 张朝印 杨伟超[1,3] 姜铭妍 徐慧[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [2]东北制药总厂,辽宁沈阳110026 [3]中国科学院研究生院,北京100049

出　　处：《生物技术》2011年第1期76-78,共3页Biotechnology

摘　　要：目的:通过太空育种的方法选育获得高活性的生黑醋酸杆菌,实现维生素C的高效发酵。方法:采用太空育种的方法,对VC一步发酵菌生黑醋酸杆菌进行神州七号搭载诱变。结果:经过多轮试管初筛、摇瓶复筛,获得1株高效菌,编号为G454。23%醇浓摇瓶发酵,发酵周期缩短约2h;在适度提高通风下,可完成高醇浓度(33%)发酵,发酵周期约24h。该菌经发酵条件优化,连续5批常规生产。结果:新菌株G454的发酵稳定性好,周期均在20h以内,平均周期18.4h;与对照相比,发酵周期缩短2h,山梨糖产率12.34mg/ml/h,较对照提高7.68%。结论:筛选所得菌株454可缩短发酵周期10%。,提高山梨糖产率7.68%。Objective：To increase the convertion rate of D-sorbitol to L-sorbose in a first-step fermentation of Vitamin C by space mutation.Method：Gluconbacter melanogenus was used in the present study.Space-flight mutation of the Gluconbacter melanogenus was carried out by Chinese spaceship＂shenzhou＂No.7.Mutants were isolated and cultured with shake-flask.Result：After preliminary and secondary screening,a strain number as G454 was obtained.The results of culture in shaking flasks showed that the average fermentation period was shorten by 2 hours than original strains under the common fermentation conditions and would accomplished the fermentation in the medium with higher concentration of D-sorbitol 33% in 24 hours.Furthermore,in 180-m3 fermention tank for 5 batches,the fermentation keep stability as well.Comparing to original strains,the fermentation period of strain G454 shorten by 2 hours and the convertion rate of L-sorbose increased by 7.68%.Conclusion：Space mutation,as a new induced mutation method,had potential advantages in breeding.Comparing with original strains,Gluconbacter melanogenus G454 is more efficient for transformantion from D-sorbitol to L-sorbose in first-step Vitamin C fermentation,which is of shorten fermentation period by 10% and higher convertion of L-sorbose by 7.68%.

关 键 词：太空诱变 选育D-山梨醇 L-山梨糖 生黑醋酸杆菌 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]