血链球菌细菌素对白色念珠菌生物膜抑制作用的研究  

Study on Biofilm Inhibit Mechanism of Streptococcus Sanguis Bacteriocin on Candida Albicans

作　　者：马晟利[1] 王琪波[2] 李旭明[2] 

机构地区：[1]黑龙江省医院口腔科,黑龙江哈尔滨150036 [2]佳木斯大学

出　　处：《口腔医学研究》2011年第2期116-118,122,共4页Journal of Oral Science Research

基　　金：黑龙江省自然科学基金(编号:D2007-03)

摘　　要：目的:提取血链球菌标准株ATCC10556的有效抗菌物质细菌素,进一步研究血链球菌细菌素对白色念珠菌生物膜的作用机理。方法:通过超声破碎、盐析、sephadex G-25过柱脱盐、透析、浓缩的方法分离血链球菌细菌素,使之作用于白色念珠菌生物膜,并在2h、6h、12h、24h、48h、72h观察白色念珠菌生物膜厚度的变化。结果:24h内白色念珠菌生物膜厚度有明显改变,12h效果最为显著。结论:血链球菌细菌素对白色念株菌生物膜具有显著的抑制作用。Objective：To extract bacteriocin effective antimicrobial substances the standard strains of Streptococcus sanguis ATCC10556 and to study the action mechanism of Streptococcus sanguis bacteriocin on Candida albicans biofilms.Methods：By ultrasonic,salt precipitation and sephadex G-25 column desalting through dialysis,sanguicin of the streptococcus bacteria isolated elements,the Candida albicans biofilms.After 2h,6h,12h,24h,48h,72h,changes observed in BF.Results：After 24h,Candida albicans biofilms changed significantly,then was 12h.Conclusion：Streptococcus sanguis bacteriocin biofilms of Candida albicans strains were significantly inhibited.

关 键 词：血链球菌 细菌素 白色念珠菌 生物膜 抑制 

分 类 号：R781.4[医药卫生—口腔医学]