适合硅橡胶膜生物反应器乙醇发酵的酵母选育  

Breeding Yeast by Acclimatization Evolution in PDMS Membrane Bioreactor

作　　者：丁文武[1] 汤晓玉[1] 傅晟伟[1] 邓克旺[1] 姜彦东[1] 原林[1] 肖泽仪[1] 

机构地区：[1]四川大学化学工程学院,四川成都610065

出　　处：《四川大学学报（工程科学版）》2011年第2期161-165,171,共6页Journal of Sichuan University (Engineering Science Edition)

基　　金：国家自然科学基金资助项目(20776088)

摘　　要：从水果中筛选出一株野生型酵母S3,并将其运用于膜生物反应器封闭循环乙醇发酵。运用酵母S3进行了3次摇瓶发酵实验,得到细胞浓度、乙醇产率以及葡萄糖转化率的最大值分别为:5.37g/L,1.90g/(L.h),87.9%;将S3菌株接入膜生物反应器封闭循环发酵系统,进行了524.4 h的封闭循环发酵实验,整个发酵过程乙醇产率为1.48g/(L.h),葡萄糖消耗速率为3.55g/(L.h),发酵结束时,细胞存活率为30%,葡萄糖转化率为81.4%,均高于菌株S0的发酵结果,乙醇-细胞比产率以及乙醇-细胞得率分别为0.148 g/(g.h)和77.63 g/g,分别是菌株S0的1.57倍和1.71倍。结果表明,酵母菌在膜生物反应器封闭循环发酵过程中有被定向驯化以适应此发酵环境的可能性,并且野生酵母S3比工业安琪酵母S0更容易被定向驯化。A yeast strain S3 screened from the fruits was applied successfully to the close-circulating ethanol fermentation in the PDMS membrane bioreactor.Three parallel pure cultures in flakes were conducted for yeast strain S3.The max cell concentration,ethanol productivity and glucose conversion were 5.37 g/L,1.90 g/（L·h）,87.9%,respectively.With inoculating S3 into the PDMS membrane bioreactor,the close-circulating ethanol fermentation was conducted,and the ferment experiment was steadily carried out for 524.4 h.The ethanol productivity was 1.48 g/（L·h）,and the glucose utilization 3.55 g/（L·h）.The yeast cell viability was still over 30% at the termination of fermentation.The glucose conversion was 81.4%,higher than that of strain S0 under similar ferment process.The ethanol yield on biomass and the specific ethanol formation rate of S3 through the ferment process were 77.63 g/g and 0.148 g/（g·h）,more 57% and 71% than those of S0,respectively.The experimental results indicated that strain S3 might be acclimatized and evolved to some extent in the process of close-circulating ethanol fermentation in the PDMS membrane bioreactor,and behave superior fermentation features to industrial Angel yeast strain S0.

关 键 词：膜生物反应器 酵母 筛选 驯化 

分 类 号：Q813[生物学—生物工程] TS262.2[轻工技术与工程—发酵工程]