EGCG对鼻咽癌CNE-2细胞增殖、凋亡及survivin、VEGF表达的影响  

Effects of EGCG on the cell proliferation, apoptosis and expressions of survivin and VEGF in nasopharyngeal carcinoma cell line CNE-2

作　　者：伍红良[1] 凌月福[1] 黄岚珍[2] 栗子芳[1] 何晓松[1] 易世江[1] 

机构地区：[1]桂林医学院附属医院耳鼻咽喉-头颈外科,541001 [2]桂林医学院科学实验中心,541004

出　　处：《实用医学杂志》2011年第7期1138-1141,共4页The Journal of Practical Medicine

基　　金：广西卫生厅科研基金资助课题(编号:重200652)

摘　　要：目的:探讨表没食子儿茶素没食子酸酯(EGCG)对鼻咽癌细胞增殖、凋亡及生存素(survivin)和血管内皮生长因子(VEGF)的影响。方法:体外培养鼻咽癌细胞,以不同质量浓度EGCG处理,采用MTT法检测细胞增殖抑制作用,Hoechst33258染色观察凋亡细胞并计算细胞凋亡率,流式细胞仪检测细胞周期变化,RT-PCR检测survivin和VEGF的表达变化。结果:EGCG处理后,CNE-2细胞增殖明显受到抑制,呈时间和剂量依赖性;CNE-2细胞凋亡率显著升高;CNE-2细胞周期阻滞于G1/G0,表现为浓度依赖性;survivin和VEGF的表达明显下调,随EGCG浓度的增加有下降趋势。结论:EGCG能抑制鼻咽癌细胞增殖、促进其凋亡;机制可能与EGCG下调survivn和VEGF的表达有关。Objective To study the effects of epigallocatechin-3-gallate （EGCG） on the proliferation, apoptosis and expressions of survivin and VEGF in nasopharyngeal carcinoma cell line CNE-2. Methods Nasopharyngeal carcinoma cells was cultured in vitro, after treated with various concentrations of EGCG, the cell proliferation ability was detected by MTF; Apoptosis of cells was observed by Hoechst staining with apoptosis rate counted;Flow cytometry was used to analyze the changes of cell cycle. RT-PCR was applied to detect the expression of survivio and VEGF. Results EGCG could effectively inhibit the growth of CNE-2 cells, arrest the cells in GI phase in a dose-dependent manner and induce cell apoptosis. Furthermore, EGCG could down-regulate the expressions of survivin and VEGF after treated with EGCG. Conclusions EGCG could significiantly suppress the proliferation and induce apoptosis in uasopharyngeal carcinoma cell line CNE-2; The possible mechanisms may be involved with decreasing the expressions of survivin and VEGF.

关 键 词：鼻咽肿瘤 表没食子儿茶素没食子酸酯 细胞系 培养的 存活素 血管内皮生长因子 

分 类 号：R739.63[医药卫生—肿瘤]