Hedgehog信号通路蛋白在结直肠黑变病及癌肿的检测分析  

Expression of hedgehog signaling pathway proteins in melanosis coli and colorectal cancer

作　　者：翁子毅[1,2] 子树明[2] 杨明[2] 杜鹏[2] 崔龙[2] 于恩达[1] 

机构地区：[1]第二军医大学附属长海医院肛肠外科,上海市200433 [2]上海交通大学医学院附属新华医院肛肠外科,200092

出　　处：《实用医学杂志》2011年第7期1192-1194,共3页The Journal of Practical Medicine

摘　　要：目的:探讨Hedgehog(Hh)信号通路活化在结肠黑变病(melanosiscoli,MC)和结直肠癌(colorectalcancer,CRC)中的作用。方法:免疫组化检测正常结直肠黏膜、MC和CRC等组织中Hh信号通路蛋白SHH、PTCH和GLI1的表达。结果:正常结直肠组织中,SHH、PTCH和GLI1蛋白不表达或轻度表达。MC中,SHH呈中到重度表达,PTCH为轻度到中度表达,而GLI1不表达或轻度表达。CRC中,SHH和PTCH多呈中到重度表达,GLI1则为轻到中度表达。不同结直肠病变的SHH(P=0.000)、PTCH(P=0.001)和GLI1(P=0.026)蛋白表达差异有统计学意义。结论:Hh信号通路在MC和CRC组织中存在相似不同程度的异常活化,提示MC可能是潜在的CRC癌前病变。Objective To study the expression of hedgehog （Hh） signal pathway proteins SHH, PTCH, and GLI1 in melanosis coli （MC） and eolorectal cancer （CRC）. Methods The expressions of SHH, PTCH, and GLI1 in the specimens of normal eolorectal mucosa, MC and CRC were detected by immunohistochemistry. Results In normal colorectal mueosa, SHH, PTCH, and GLI1 were absent or slightly expressed. In MC, SHH was strongly expressed, PTCH was expressed slightly or medially, and GLI1 was absent or only slightly expressed. In CRC, SHH and PTCH were strongly expressed, while GLI1 was expressed slightly or medially. The expressions of SHH （P = 0.000）, PTCH （P = 0.001）, and GLI1 （P = 0.026） were significantly different among different colorectal diseases. Conclusion Hh signal pathway proteins were similarly abnormal activated in varying degrees between MC and CRC, indicating that MC may be a potential precancerous lesion of CRC.

关 键 词：结直肠肿瘤 结肠黑变病 SHH PTCH GLI1 

分 类 号：R735.3[医药卫生—肿瘤]