N-辛基-β-D-吡喃葡萄糖苷与BSA相互作用的研究  

Studies on the Interaction between N-Octyl-β-D-glucopyranoside and Bovine Serum Albumin

作　　者：郑云[1] 陈得军[1] 王公轲[1] 徐梅花[1] 卢雁[1] 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007

出　　处：《化学学报》2011年第6期633-639,共7页Acta Chimica Sinica

基　　金：国家自然科学基金(No.20673034)资助项目

摘　　要：通过稳态荧光法、紫外-可见光谱法和表面张力法研究了N-辛基-β-D-吡喃葡萄糖苷(OGP)与牛血清白蛋白(BSA)在缓冲液中的相互作用.根据OGP溶液和OGP/BSA混合溶液的表面张力曲线可以看出,蛋白质的加入改变了单一表面活性剂溶液的表面张力曲线.蛋白质的加入还使混合体系的临界胶束浓度(cmc*)大于单一表面活性剂的临界胶束浓度(cmc),这主要是由于OGP与蛋白质结合减少了单体OGP分子的浓度所致.加入荧光探针芘测量了OGP和OGP/BSA溶液的Ⅰ1/Ⅰ3值,结果也表明BSA的加入增大了OGP的聚集浓度,其原因与表面张力的变化原因是相同的.在OGP/BSA体系中,随着OGP浓度的增大,紫外吸收减弱、荧光强度有规律的降低,而且荧光发射峰位发生蓝移,说明它们的结合部位趋向于Trp残基上;同时通过同步荧光和Ⅰ-猝灭实验,进一步证明了OGP与BSA的结合部位是在BSA的疏水空腔内的色氨酸残基上.The interactions between N-Octyl-β-D-glucopyranoside（OGP） and bovine serum albumin（BSA） have been investigated by fluorescence,ultraviolet spectroscopy and surface tension measurements.The surface tension curve of the OGP solution can be modified by the addition of BSA.From the surface tension curve of the OGP solution and OGP/BSA solution,we can see that the critical micelle concentration of OGP in OGP/BSA solution is bigger than in OGP solution.This is because that the concentration of OGP monomer is decreased by the binding of OGP molecules on the surface of BSA.It was found that the pres-ence of protein delays the surfactant aggregation by using the pyrene 1∶3 ratio method.The reason is same with that of the surface tension change.The fluorescence intensity and ultraviolet absorption intensity of BSA gradually decrease upon increasing the concentration of OGP,and a blue shift of the max fluorescence emission wavelength of BSA was observed,this indicated that OGP mainly interacts with Trp residues compared to Tyr residues.The results from synchronous fluorescence spectrum and Ⅰ-quenching experi-ments also indicated that OGP mainly interacts with Trp residues of hydrophobic loop.

关 键 词：N-辛基-β-D-吡喃葡萄糖苷 表面张力 荧光 紫外 牛血清白蛋白 

分 类 号：Q503[生物学—生物化学]