注射用脑蛋白水解物对大鼠肾上腺嗜铬细胞瘤细胞增殖与氧化损伤的影响  

Effects of cerebroprotein hydrolysate for injection on PC12 pheochromocytoma cells proliferation and oxidative damage

作　　者：蒋玉辉[1] 梁蔚阳[1] 

机构地区：[1]广东省药品检验所,广东广州510180

出　　处：《中国医药导报》2011年第10期30-31,共2页China Medical Herald

摘　　要：目的:探讨注射用脑蛋白水解物对大鼠肾上腺嗜铬细胞瘤细胞的增殖与过氧化氢氧化损伤的影响。方法:采用MTT法,观察不同浓度的注射用脑蛋白水解物对体外培养的大鼠肾上腺嗜铬瘤细胞(PC12)生长及过氧化氢损伤保护的作用。结果:注射用脑蛋白水解物对PC12细胞的增殖及氧化损伤的保护作用存在量效关系。结论:注射用脑蛋白水解物可促进PC12细胞生长,并可保护细胞减轻过氧化氢的损伤。Objective：To explore the biological effects of cerebroprotein hydrolysate for injection on the proliferation and oxidative damaged rat pheochromocytoma cells（PC12）.Methods：Thiazoly blue（MTT） colorimetric assay was used to determine the proliferation and oxidative damaged protection of PC12 cells after different doses of cerebroprotein hydrolysate for injection were added into the culture medium.Results：The proliferation and protection effects on PC12 cells of cerebroprotein hydrolysate for injection were depended on its doses.Conclusion：Cerebroprotein hydrolysate for injection may promote the proliferation and protect the PC12 cells injured from oxidation.

关 键 词：注射用脑蛋白水解物:大鼠肾上腺嗜铬瘤细胞 MTT 

分 类 号：R781.4[医药卫生—口腔医学]