前列腺癌中RhoC和Rho激酶1的表达及其对丝裂原活化蛋白激酶和Akt蛋白的影响  

Expression of RhoC and ROCK-1 and their effects on MAPK and Akt proteins in prostate carcinoma

作　　者：卜强[1] 汤华明[2] 谈健[2] 胡潇[2] 王东文[1] 

机构地区：[1]山西医科大学第一医院泌尿外科,太原030001 [2]江苏大学附属人民医院泌尿外科

出　　处：《中华肿瘤杂志》2011年第3期202-206,共5页Chinese Journal of Oncology

基　　金：江苏省卫生厅资助项目（P200956）

摘　　要：目的检测RhoC和Rho激酶1（ROCK-1）在前列腺癌中的表达，探讨RhoC和ROCK-1在前列腺癌形成过程中的可能作用。方法收集73例前列腺癌患者穿刺活检或手术切除的癌组织和对应的癌旁组织。采用逆转录聚合酶链反应（RT—PCR）检测RhoC mRNA和ROCK-1 mRNA表达，采用Western blot和免疫组化法检测RhoC和ROCK-1蛋白的表达。构建RhoC基因真核表达载体，转染前列腺癌不转移亚系PC-3M-284，并检测丝裂原活化蛋白激酶（MAPK）和Akt磷酸化水平变化。结果前列腺癌组织中，RhoC mRNA和ROCK-1 mRNA的阳性率分别为72．6％（53／73）和68．5％（50／73），癌旁组织分别为34．2％（25／73）和38．4％（28／73），差异均有统计学意义（均P〈0．01）。RhoC mRNA和ROCK-1 mRNA的表达与肿瘤转移呈正相关，而与肿瘤的Gleason分级无关。RhoC和ROCK-1蛋白在前列腺癌组织中的表达高于癌旁组织，并且与磷酸化MAPK和磷酸化Akt的表达呈正相关。携带RhoC基因的载体转染PC-3M-284细胞后，可以上调MAPK和Akt的磷酸化表达水平。结论RhoC和ROCK一1在前列腺癌组织中的表达高于癌旁组织，且与肿瘤转移相关。RhoC和ROCK一1可能通过活化MAPK和Akt参与了前列腺癌的形成、浸润和转移。Objective To detect the expression of RhoC and Rho kinase 1 （ROCK-1） in prostate carcinoma, and explore the possible mechanism of RhoC/ROCK-1 in the pathogenesis of prostate carcinoma. Methods Tissue specimens from 73 patients with prostate carcinoma and corresponding paracancerous tissues were obtained by prostate cancer biopsy or radical prostatectomy. The expression of RhoC/ROCK-1 mRNA was detected by RT-PCR. Western blot and immunohistochemistry were performed to derteet the expression of RhoC/ROCK-1 protein. Eukaryotic expression plasmids of RhoC were constructed and transfected into PC-3M-21M cells, p-MAPK and p-Akt were detected by Western bolt. Results The expression levels of RhoC and ROCK-1 mRNA in the prostate carcinomas were significantly higher than those in corresponding paracancerous tissues [72.6% （53/73） vs. 34.2% （25/73） ;68.5% （50/73）vs. 38.4% （28/73）, P 〈0.01 ], respectively. The results indicated that RhoC/ROCK-1 mRNA expression had no significant correlation with Gleason grade. However, the expression of RhoC/ROCK-1 mRNA showed a significant positive correlation with distant metastasis. The RhoC/ROCK-1 protein expression in prostate cancer was also higher than corresponding paracancerous tissues, and showed a significant positive correlation with p-MAPK and p-Akt expression levels. In addition, p-MAPK and p-Akt expression levels were up-regulated in the transcripts. Conclusion Expression levels of RhoC and ROCK-1 in prostate carcinoma are higher than those in corresponding paracaneerous tissues, showing a significant positive correlation with distant metastasis. RhoC/ROCK-1 may be involved in the development, invasion and metastasis of prostate carcinoma.

关 键 词：前列腺肿瘤 RHOC Rho激酶1 丝裂原活化蛋白激酶 AKT 浸润 转移 

分 类 号：R737.25[医药卫生—肿瘤]