HPLC同时检测食品接触材料中三聚氰胺与三聚氰酸单体迁移量  

Simultaneous determination of melamine and cyanuric acid in food contact material by HPLC

作　　者：井伟[1,2] 陈旻实[2] 李小晶[2] 吕水源[2] 陈锦东[2] 吴洪成[2] 郑思远[2] 陈锦权[1] 

机构地区：[1]福建农林大学食品科学学院,福州350002 [2]福建出入境检验检疫局技术中心,福州350001

出　　处：《分析试验室》2011年第4期34-38,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20905013);福建省科技计划重点项目(2009Y0001)资助

摘　　要：建立了一种用液相色谱技术检测分析食品接触材料中三聚氰胺与三聚氰酸单体迁移量的方法。样品采用水、4%乙酸和10%乙醇3种模拟物进行浸泡。色谱分离采用Phenomenex Luna NH2柱,柱温:30℃,流动相为乙腈-5 mmol/L,pH=6.5 PBS缓冲溶液(75∶25,V/V),流速1.0 mL/min,DAD检测波长为214 nm。在优化条件下,三聚氰胺和三聚氰酸分别在0.1～110 mg/L和0.3～135 mg/L范围内,峰面积和质量浓度线性关系良好。在0.5 mg/L的添加水平下,三聚氰胺和三聚氰酸的平均回收率分别为101.7%～102.2%和88.0%～88.9%,相对标准偏差分别为2.45%～3.42%和3.54%～4.10%,检出限分别为0.02 mg/L和0.08 mg/L。方法可同时测定食品接触材料中三聚氰胺与三聚氰酸单体迁移量。A method was developed for the determination of melamine and cyanuric acid by HPLC.Samples were extracted with H2O,4% acetic acid,10% ethanol.The separation was performed on a Phenomenex Luna NH2 column at 30 ℃ with the mobile phase（acetonotrile/PBS（5 mmol/L,pH=6.5） 75∶25 V：V） at a flow rate of 1.0 mL/min with detection at 214 nm.Linear responses were observed for samples in the ranges from 0.1 to 110 mg/L and from 0.3 to 135 mg/L.The method provided recoveries of 101.7%~102.2% and 88.0%~88.9% in the concentration of 0.5 mg/L for melamine and cyanuric acid,RSDs were 2.45%~3.42% and 3.54%~4.10%,respectively.The LODs were 0.02 mg/L for melamine and 0.08 mg/L for cyanuric acid.

关 键 词：三聚氰胺 三聚氰酸 食品接触材料 高效液相色谱 迁移 

分 类 号：O657.7[理学—分析化学]