工程菌所产黑色素对苏云金芽孢杆菌毒力的保护作用  

Protection of Bacillus thuringiensis toxicity by melanin from engineering bacteria

作　　者：胡海艳[1] 黄雅莉[1] 梁志洲[1] 周世宁[1] 

机构地区：[1]中山大学生命科学学院/有害生物防治与生物资源利用国家重点实验室,广州510275

出　　处：《农药学学报》2011年第2期205-208,共4页Chinese Journal of Pesticide Science

基　　金：国家高技术研究发展计划(863计划)项目(2008AA09Z402);广州市科技计划项目(2088J1-C241)

摘　　要：对基因工程菌Escherichia.coli fss6所产黑色素进行了紫外保护研究。结果表明:添加黑色素的苏云金芽孢杆菌(Bt)经紫外照射后菌体的存活率是不加黑色素菌体的8倍;黑色素能有效保护Bt的晶体毒素蛋白免受紫外照射的降解;随着被照射菌体中黑色素浓度的增加,其杀虫活性也相应提高。The protection of the ability of Bacillus thuringiensis against ultraviolet irradiation by melanin produced by engineering bacterium Escherichia coli fss6 was studied.Results showed that the survival ratio of the B.thuringiensis under the protection of melanin was 8 times higher than that of those without melanin after UV irradiation.Melanin could protect the toxin protein from UV degradation significantly,the insecticidal activity of B.thuringiensis was improved as the concentration of melanin increased.

关 键 词：黑色素 苏云金芽孢杆菌 紫外保护 活性检测 

分 类 号：TS264.4[轻工技术与工程—发酵工程]