应用间期FISH研究在肾癌石蜡包埋组织中抽提细胞核的染色体异常  

USING INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION TO STUDY RENAL CELL CARCINOMA IN EXTRACT NUCLEI FROM PARAFFIN-EMBEDDED TISSUE

作　　者：邹泓[1] 杨壹羚[2] 李露霞[1] 赵瑾[1] 齐妍[1] 梁伟华[1] 郭长军[1] 李锋[1] 

机构地区：[1]新疆石河子大学医学院病理教研室,石河子832002 [2]天津医科大学附属肿瘤医院乳腺病理室

出　　处：《现代预防医学》2011年第7期1304-1306,共3页Modern Preventive Medicine

基　　金：国家自然科学基金(30560169);石河子大学高层次人才科研启动基金(RCZX200784);石河子大学"263"青年骨干教师资助项目(YX06016)

摘　　要：[目的]应用间期FISH研究从石蜡包埋肾癌组织中抽提细胞核的染色体异常,探讨此方法的可行性。[方法]选择20例不同类型的肾癌(8例乳头状肾癌,4例Xp11.2染色体异位性肾癌,6例肾透明细胞癌,2例肾嗜酸细胞腺瘤)的石蜡包埋组织,抽提间期细胞核,制备单细胞悬液,用CEP17探针检测17号染色体的倍体,验证此方法的有效性。[结果]在全部20例肾癌样本中成功进行了FISH检测,其中,8例乳头状肾癌存在17号染色体的三体,2例肾嗜酸性腺瘤与6例肾透明细胞癌中17号染色体倍体正常,4例Xp11.2易位性肾癌存在17号染色体的3倍与4倍体。[结论]在石蜡包埋组织中抽提间期细胞核,应用FISH检测染色体异常的方法敏感、特异、可行。[Objective]To use interphase fluorescence in situ hybridization（FISH）to study renal cell carcinoma（RCC）in extract isolated nuclei from paraffin-embedded tissue and investigate its efficacy.[Methods]The efficacy of this method was investigated in 8 papillary renal cell carcinoma（PRCC）,4 Xp11.2 translocations RCC（Xp11.2RCC）,6 clear cell carcinoma（CCRCC）,and 2 renal oncocytoma.Using interphase FISH with CEP17 probe to study chromosomal 17 ploid in extract isolated nuclei from formalin fixed and paraffin embedded tissue.[Results]FISH studies were successful for each of the 20 specimens.Triploidy in chromosome 17 were detected in all specimens of PRCC,dipoid in chromosome 17 were detected in 8 specimens of oncocytoma,and polyploid in chromosome 17 were detected in 4 specimens of Xp11.2RCC.[Conclusion]The FISH technique is useful to detect chromosome anomalies with high sensitivity and specificity in extracted nuclei from paraffin-embedded tissue.

关 键 词：荧光原位杂交 细胞核抽提 染色体倍体 17号染色体 

分 类 号：R737.11[医药卫生—肿瘤]