苦荞转录因子基因FtMYB1的克隆及序列分析  

Clone and Sequence Analysis of a Transcription Factor Gene FtMYB1 from Fagopyrum tataricum

作　　者：白悦辰[1] 陶亮[1] 蒙华[1] 李成磊[1] 陈惠[1] 吴琦[1] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《四川农业大学学报》2011年第1期16-21,共6页Journal of Sichuan Agricultural University

基　　金：四川农业大学"双支计划"

摘　　要：采用RACE-PCR技术,从苦荞花蕾克隆得到一个MYB基因(FtMYB1)。结果表明,FtMYB1基因DNA序列全长863bp(GenBank,JF313344),含两个内含子,内含子1长度为78bp(134～211bp),内含子2长度为74bp(342～415bp);cDNA序列全长711bp(GenBank,JF313345);开放阅读框(ORF)编码236个氨基酸,理论分子量26.9kDa,等电点6.33。序列比对及同源建模分析表明,FtMYB1蛋白与其他植物MYB结合域有高度的同源性,具有MYB同源蛋白的典型特征。A transcription factor gene FtMYB1 was isolated from the flower buds of Fagopyrum tataricum by RACE-PCR method.The results showed that the full-length DNA of FtMYB1 was 863 bp（GenBank,JF313344） with two introns,78 bp intron-1（134-211 bp） and 74 bp intron-2（342-415 bp）.Meanwhile,the full-length cDNA of FtMYB1 was 711 bp（GenBank,JF313345）.The open reading frame（ORF） of FtMYB1 encodes 236 amino acid residues with a predicted molecular mass of 26.9 kDa and an isoelectric point of 6.33.Sequence analysis and homology modeling have suggested that FtMYB1 shows high homology with other botanic MYB.

关 键 词：苦荞 MYB转录因子 基因克隆 序列分析 

分 类 号：S517[农业科学—作物学]