K-ras基因突变表达谱与结直肠癌肝转移的相关性分析  

Correlation analysis between K-ras gene mutation and hepatic metastases from colorectal cancer

作　　者：王涛[1] 王国庆[1,2] 王权[1] 张洋[1] 所剑[1] 

机构地区：[1]吉林大学白求恩第一医院胃肠外科,吉林长春130021 [2]吉林大学白求恩医学院

出　　处：《中国实验诊断学》2011年第3期449-452,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨K-ras基因突变谱与结直肠癌肝转移的相关性,为进一步阐明结直肠癌肝转移的分子机制提供理论依据。方法收集2008年1月至2010年8月在我院治疗的结直肠癌肝转移患者30例,进行K-ras基因突变检测,分析K-ras基因的突变类型及频率,探讨K-ras基因的突变谱与结直肠癌肝转移患者性别、年龄、原发灶位置、转移位置、病灶形态及TNM分期的相关性。结果 30例结直肠癌肝转移患者组织中K-ras基因突变者14例,突变率为47%,其中12密码子突变9例,突变率为30%;13密码子突变者5例,突变率为17%。突变表达谱在性别、年龄、原发部位、肝脏转移灶部位、病灶形态、TNM分期方面差异无统计学意义(P>0.05)。结论 K-ras基因在结直肠癌肝转移的患者中突变率较高,以12,13号密码子突变最为常见,突变类型及频率在性别、年龄、原发部位、肝脏转移灶部位、病灶形态、TNM分期方面无显著性差异。Objective TO evaluate the correlation between K-ras gene mutation and hepatic metastases from colorectal cancer for supplying theoretical support for further illustration on molecular mechanisms.Methods Clinicopathological data of 30 cases with hepatic metastases from colorectal cancer from Jan.2008 to Aug.2010 in our department were collected to set up the database.All the specimens are DNA sequenced by pyrosequencing technology in order to detect k-ras gene mutation type and mutation location.Then the associations between K-ras mutation status and clinicopathological characteristics in colorectal cancer were analyzed.Results 14 cases were detected mutation in 30 cases of colorectal cancer specimens.The mutation rate was 47%.There were 9 cases of mutation occurred in codon 12,5 cases occurred in codon 13.There were no significant differences in gender,age,location of primary tumor,hepatic metastases location,tumor form,and TNM stage.Conclusion The frequency of K-ras mutation is no correlated with gender,age,location of primary tumor,hepatic metastases location,tumor form,and TNM stage.

关 键 词：K-RAS基因 结直肠癌 肝转移 基因突变 

分 类 号：R735.3[医药卫生—肿瘤]