酶法提取马齿苋多糖  

Enzyme Extraction of Polysaccharide from Portulaca oleracea L.

作　　者：钱志伟[1] 王会晓[2] 杨海蛟[1] 

机构地区：[1]河南农业职业学院,郑州451450 [2]郑州轻工业学院食品与生物工程学院,郑州450002

出　　处：《中国农学通报》2011年第5期475-478,共4页Chinese Agricultural Science Bulletin

摘　　要：为了优化马齿苋多糖的提取工艺,将纤维素酶用于提取马齿苋多糖,在单因素实验的基础上,采用L9(34)正交实验法对酶法提取马齿苋多糖的条件进行优化,考察酶用量、酶解温度、pH、酶解时间对马齿苋多糖提取得率的影响。结果表明:最合适的工艺参数为酶用量2.0%、酶解温度40℃、pH6.0、酶解时间110min。该条件下,马齿苋多糖得率达到9.7%。酶法提取纤维素多糖工艺简洁,能量消耗减少,是一种提取马齿苋多糖的的适宜方法。In order to extraction of polysaccharide from Portulaca oleracea L.. The optimum enzyme extraction technology was optimized with L 9 （3 4 ） orthogonal test. The influence of enzyme dosage,extraction temperature , pH and extraction time on yield of polysaccharide was studied. The optimum conditions were as follows： dosage of cellulose 2.0%, temperature 40℃, time 110 min, pH 6.0 and extraction yield were 9.7%. It indicated that enzyme extraction was a suitable method for extraction polysaccharide from Portulaca oleracea L. for it was simple and has shorter extraction time.

关 键 词：酶 提取 马齿苋 多糖 

分 类 号：TS201.1[轻工技术与工程—食品科学]