重组草酸脱羧酶的表达及酶学性质研究  

Expression and Characterization of Oxalate Decarboxylase

作　　者：林日辉[1] 许丽莉[1] 农勉[1] 张陆成[1] 

机构地区：[1]广西民族大学化学与生态工程学院,化学与生物转化过程新技术广西高校重点实验室,广西南宁530006

出　　处：《食品与发酵工业》2011年第2期57-61,共5页Food and Fermentation Industries

基　　金：广西民族大学重点项目科研基金(200701YJ01);化工过程创新技术研发平台建设(桂科能0992028-13)资助课题

摘　　要：研究了重组草酸脱羧酶的表达及其酶学性质。经IPTG诱导，每克湿重菌体收获草酸脱羧酶活力820U，经Ni—NTA亲和层析酶液纯化2．07倍，酶活力回收60．38％。酶促反应的最适温度为50～55℃，最适pH值为3．5，添加EDTA及Fe^2＋对酶活力有促进作用，Mn^2＋抑制酶活。在pH4．0，温度37℃下Km值为14．53mmol／l，Vmax为133．33U／mg。Oxalate decarboxylase was expressed and characterized in this paper. After induced by IPTG,820 U/ g （wet weight） of oxalate decarboxylase was obtained,and the enzyme was purified 2.07 fold by metal chelate affinity chromatography with a yield of 60.38%. The enzyme had an optimum temperature between 50 and 55 ℃ , and optimum pH at 3.5. Addition of EDTA or Fe2＋ would activate oxalate deearboxylase,but addition of Mn2. would inhibit the enzyme. Under condition of pH4.0,37 ℃ ,the enzyme＇ s Km was 14.53 mmol/1 with Vmax = 133.33 U/mg.

关 键 词：草酸脱羧酶 诱导表达 纯化 酶学性质 

分 类 号：TQ925[轻工技术与工程—发酵工程]