黏度法测定γ-聚谷氨酸含量  

Determination of γ-polyglutamic Acid Concentration by a Viscosity-assaying Method

作　　者：姚俊[1] 孙荣斌[2] 魏钦俊[1] 曹新[1] 鲁雅洁[1] 

机构地区：[1]南京医科大学基础医学院,生物技术系,江苏南京210029 [2]南京医科大学药学院,江苏南京210029

出　　处：《食品与发酵工业》2011年第2期176-178,共3页Food and Fermentation Industries

基　　金：江苏省高校自然科学研究项目(09KJB530007)

摘　　要：采用乌氏黏度计测定了不同浓度下γ-聚谷氨酸稀溶液的黏度,由哈金斯(Huggins)方程和克拉默(Kraemer)方程结合外推法求得室温下γ-聚谷氨酸在中性水溶液中的特性黏度[η]为7.830 L/g,并推导出由溶液的相对黏度ηr与增比黏度ηsp计算γ-聚谷氨酸溶液浓度的公式。依据溶液黏度与浓度的关系,可以简便、快速、准确地估算出γ-聚谷氨酸的浓度。Abstract The viscosity of γ-polyglutamic acid （γ-PGA） solution at different concentration was deter- mined by ubbelohde viscometer. The intrinsic viscosity [η] of ,γ-PGA was 7. 830 L/g calculated from Huggins ＆ Kraemer equation. Meanwhile, the correlation of relative viscosity （ηsp）, specific viscosity （ηsp） and the γ-PGA concentration in aqueous solution was deduced. It was expected that the γ-PGA concentration could be facilely and precisely attained by the viscosity-assaying method.

关 键 词：Γ-聚谷氨酸 黏度 浓度 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]