Proteomics Analysis of T Lymphocytes Damage Induced by Ionizing Irradiation  

作　　者：Shi, Ying Zhang, Xuan Liu, Ning Liang, Feng Song, Fengrui Liu, Zhiqiang Liu, Shuying[1] 

机构地区：[1]Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China [2]School of Public Health, Jilin University, Changchun, Jilin 130021, China [3]Central Laboratory, The Second Hospital of Jilin University, Changchun, Jilin 130041, China [4]College of Pharmacy, Jilin University, Changchun, Jilin 130021, China [5]Graduate School of the Chinese Academy of Sciences, Beijing 100039, China

出　　处：《Chinese Journal of Chemistry》2011年第1期159-164,共6页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （Nos. 20675079, 30672600）.

摘　　要：Human T lymphocytes were found to be highly radiosensitive and complex cellular responses including apoptosis could be induced upon exposure to X-ray irradiation. However, the mechanism of apoptosis associated with irradiation was not clear. In this study, a proteomic method was applied to investigation on alteration of proteome of human T-lymphocyte cells after irradiation. The Jurkat cells were irradiated with 4 Gy X-ray and the cell lysates were collected at different times after irradiation （6, 12, 18, 24 and 48 h）. The whole proteins were separated and quantified by two-dimensional fluorescence difference gel electrophoresis, and then the differentially expressed proteins were identified by mass spectrometry. 4 proteins exhibited significant irradiation-induced difference in abundance, including L-plastin, bifunctional purine biosynthesis protein, tubulin beta chain, beta-actin. Differentially expressed proteins were reported to be directly or indirectly involved in the function of human T lymphocyte. Thus, this study might provide clues to identify proteins with biological significance related to irradiation.Human T lymphocytes were found to be highly radiosensitive and complex cellular responses including apoptosis could be induced upon exposure to X-ray irradiation. However, the mechanism of apoptosis associated with irradiation was not clear. In this study, a proteomic method was applied to investigation on alteration of proteome of human T-lymphocyte cells after irradiation. The Jurkat cells were irradiated with 4 Gy X-ray and the cell lysates were collected at different times after irradiation （6, 12, 18, 24 and 48 h）. The whole proteins were separated and quantified by two-dimensional fluorescence difference gel electrophoresis, and then the differentially expressed proteins were identified by mass spectrometry. 4 proteins exhibited significant irradiation-induced difference in abundance, including L-plastin, bifunctional purine biosynthesis protein, tubulin beta chain, beta-actin. Differentially expressed proteins were reported to be directly or indirectly involved in the function of human T lymphocyte. Thus, this study might provide clues to identify proteins with biological significance related to irradiation.

关 键 词：T-LYMPHOCYTES IRRADIATION ELECTROPHORESIS APOPTOSIS PROTEOMICS L-PLASTIN 

分 类 号：Q51[生物学—生物化学] S852.4[农业科学—基础兽医学]