鳜鱼与鲢鱼肌球蛋白轻链2启动子的克隆及其序列比较分析  

Cloning and Sequence Analysis of the Myosin Light Chain 2 Gene Promoter Region from Siniperca Chuasti and Mylopharyngodon Piceus

作　　者：赵发兰[1,2] 周瑞雪[1] 成嘉 陈敦学[1,2] 农小献[1,2] 蒙涛[1] 张建社[1] 褚武英[1,1] 

机构地区：[1]长沙大学生物工程与环境科学系,中国长沙410003 [2]广西师范大学生命科学学院,中国桂林541004

出　　处：《湖南师范大学自然科学学报》2011年第1期75-79,共5页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金资助项目(30972263);湖南省教育厅重点资助项目(09A008);长沙学院引进人才科研资助项目(SF080401);“湖南省高校科技创新团队支持计划”资助项目(湘教通[2008]49号)

摘　　要：采用PCR方法从鳜鱼(Siniperca chuasti)基因组DNA中分离得到了鳜鱼肌球蛋白轻链2基因启动子序列,其大小为890 bp,包含1个TATA框、3个MEF2结合位点和5个与b HLH转录因子相结合的E-框(CANNTG).用同样的方法从鲢鱼基因组DNA中分离得到鲢鱼肌球蛋白轻链2基因启动子序列,其大小为870bp,包含了1个TATA框、1个MEF2结合位点和3个与bHLH转录因子相结合的E-框.鳜鱼比鲢鱼肌球蛋白轻链2基因启动子转录调控元件更加丰富.The myosin light chain 2 promoter was isolated from Siniperca chuasti genomic DNA by polymerase chain reaction（PCR） and its contains 890 base pairs,including 1 TATA box,3 MEF2 binding sites and 5 E-box（CANNTG）,where the bHLH transcription factors were combined.The Mylopharyngodon piceus myosin light chain 2 promoter was also isolated from its genomic DNA by polymerase chain reaction（PCR）,containing 870 base pairs,including 1 TATA box,1 MEF2 binding site and 3 E-box（CANNTG） where the bHLH transcription factors were combined.Distance of start sites of the proximal MEF2 binding sites and TATA-box of MLC2F promoter was 28 bp and 21 bp in the Siniperca chuasti and the Mylopharyngodon piceus,respectively.The comparative analysis of MLC2 promoters of the two fish species revealed the key regulatory domain for gene expression and provided us better understanding of MLC2 functions in fish.

关 键 词：鳜鱼 鲢鱼 肌球蛋白轻链2 启动子 序列分析 

分 类 号：Q785[生物学—分子生物学]