低能离子注入法选育透明质酸高产菌株  

Screening of High-yield Hyaluronic Acid-producing Strain by Low Energy Ion Implantation

作　　者：张伟[1,2] 姚俊[1] 陈宽婷[1] 孙荣斌[3] 魏钦俊[1] 曹新[1] 

机构地区：[1]南京医科大学基础医学院生物技术系,江苏南京210029 [2]江苏省常熟职业教育中心校旅游管理系,江苏常熟215500 [3]南京医科大学药学院,江苏南京210029

出　　处：《食品科学》2011年第7期269-272,共4页Food Science

基　　金：江苏省高校自然科学研究计划项目(09KJB530007)

摘　　要：以一株兽疫链球菌(Streptococcus zooepidemicus)NN-7为出发菌株,利用30keV,剂量为8×1014N+/cm2氮离子进行诱变,筛选获得溶血素和透明质酸酶双缺陷型突变菌株,经摇瓶发酵培养筛选获得一株透明质酸高产菌株SH-9,透明质酸产量达3.23g/L,相对分子质量达2.12×106,相对于原始菌株,透明质酸产量及相对分子质量分别提高了104.4%及35.9%。6代传代实验表明诱变后得到的高产透明质酸菌株具有较好的传代稳定性。低能离子注入法可作为有效的诱变手段用于透明质酸高产菌株的选育。Streptococcus zooepidemicus NN-7 was subjected to mutagenesis by means of low energy ion implantation of 8×1014 N＋/cm2 dose at 30 keV.After culture in selective medium,the mutant strains with double deficiency of hemolysin and hyaluronic acid-degrading enzyme were screened.Among these strains,a mutant strain capable of producing a high yield of hyaluronic acid was selected and designated as SH-9.The strain still revealed excellent passage stability after six generations.The yield of hyaluronic acid reached up to 3.23 g/L with determined relative molecular weight（Mr） of 2.12×106.Compared with the original parent strain,the yield and molecular weight of hyaluronic acid derived from SH-9 were increased by 104.4%and 35.9%,respectively.

关 键 词：透明质酸 兽疫链球菌 离子注入 诱变选育 

分 类 号：Q933[生物学—微生物学]