利用96孔板和酿酒酵母生长圈复合筛选高产管囊酵母  

Compound Screening High-yield Pachysolen tannophilus by Use of 96-well Plates and Growth Cycle of Saccharomyces cerevisiae

作　　者：蔡万华[1] 丁少鹏[1] 叶蕊芳[1] 耿丽云[1] 倪琳[1] 苏阳措[1] 

机构地区：[1]华东理工大学生物反应器国家重点实验室,上海200237

出　　处：《酿酒科技》2011年第4期36-38,共3页Liquor-Making Science & Technology

摘　　要：野生酿酒酵母不能利用木糖作为碳源,但是可以利用乙醇作为碳源和能源。管囊酵母可以利用木糖生产乙醇。酿酒酵母可以利用管囊酵母生产的乙醇作为碳源。本研究将管囊酵母进行紫外诱变后,利用96孔板培养,选取OD值较高的孔进行酿酒酵母生长圈筛选。管囊酵母在木糖(5%)为唯一碳源的YNB培养基上生长并产生乙醇。产生的乙醇越多,指示菌酿酒酵母的生长圈相对越大。利用酿酒酵母生长圈法筛选的正变率为35.22%,筛出1株菌UV171,乙醇产量为10.7 g/L,比对照高25.9%,对菌株UV171进行稳定性实验,传至3代其产量稳定。Wild Saccharomyces cerevisiae can not use xylose as carbon source but it can use ethanol as carbon source and energy.Pachysolen tannophilus can ferment xylose to produce ethanol.Furthermore,Saccharomyces cerevisiae can use the produced ethanol as carbon source.In this study,after UV mutation of Pachysolen tannophilus,96-well plates was used to select the microorganism of high OD,and then the screening of high-yield Pachysolen tannophilus was carried out by growth cycle of Saccharomyces cerevisiae experiment.Pachysolen tannophilus grew and produced ethanol in YNB medium（xylose（5 %）as the sole carbon source）.The more ethanol produced,the growth circle of Saccharomyces cerevisiae was larger.The positive rate of such method was 35.22 %.And the strain UV171 was eventually screened out with its ethanol-producing capacity as 10.7 g/L（25.9 % higher than the control strains）.Besides,its stability experiments proved that its yield was stable after 3 passage.

关 键 词：微生物 管囊酵母 96孔板培养 酿酒酵母生长圈 木糖乙醇发酵 

分 类 号：TS261.1[轻工技术与工程—发酵工程]