顶空静态进样GC-MS分析羌活挥发性成分  

GC-MS analysis for volatile compounds in Rhizoma et Radix notopterygii by headspace injection

作　　者：蒋海强[1] 张莹[1] 容蓉[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《药物分析杂志》2011年第4期735-738,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家重点基础研究发展计划("973"计划)资助项目(2007CB512601)

摘　　要：目的:建立顶空静态进样快速分析羌活挥发性成分的方法。方法:采用顶空静态进样技术提取羌活挥发性成分,对其进行气相色谱-质谱(GC-MS)结合保留指数双重分析。结果:通过GC-MS及保留指数双重定性,鉴定出羌活中24个挥发性化学成分,其中α-蒎烯、β-蒎烯、3-蒈烯、D-柠檬烯4种成分约占羌活挥发性化学成分的82.6%。结论:顶空静态进样技术结合GC-MS及保留指数定性,可以方便、快捷地提供羌活中挥发性成分的化学信息。Objective：To establish a rapid analytical method for volatile components in rhizoma et radix notopterygii.Methods：The volatile components were extracted by head-space injection technique and analyzed by gas chromatography-mass spectrometry（GC-MS） combined with Kovats retention indices.Results：Among the volatile components that detected,36 compounds were identified by GC-MS combined with Kovats retention indices;the content of four volatile compounds,including α-pinene,β-pinene,3-carene and D-limonene,can reach up to 81% of the total.Conclusions：GC-MS and Kovats retention indices combined with static headspace injection technique can be used as a rapid,direct and convenient analytical method for the volatile components in Chinese herbal medicine.

关 键 词：羌活 挥发性成分 顶空静态进样技术 气相色谱-质谱联用 保留指数 

分 类 号：R917[医药卫生—药物分析学]