Cyt b和12S rRNA基因进化速度对构建系统进化树的影响  

Effects of Evolution Rate of Cyt b,12S rRNA Genes on Constructing Phylogenetic Trees

作　　者：王利利[1] 彭建军[1] 于冬梅[1] 胡诗佳[1] 高赛飞[1] 傅美兰[1] 刘曦庆[1] 

机构地区：[1]华南濒危动物研究所物种鉴定中心,广东广州510260

出　　处：《安徽农业科学》2011年第9期5101-5103,5106,共4页Journal of Anhui Agricultural Sciences

基　　金：广东省自然科学基金项目(9151026001000003);广东省科学院优秀青年科技人才基金项目(200804);广东省野生动物保护和利用公共实验室基金项目(200901)

摘　　要：[目的]论证线粒体Cyt b和12S rRNA基因不同进化速率影响构建的系统树拓扑结构。[方法]从GenBank下载了蛇亚目12科15种蛇线粒体全序列,提取Cyt b和12S rRNA基因序列,选用GTR+I+G核苷酸替代模型,基于最大似然法,用PAUP4.0软件分别构建2个基因的系统关系树。[结果]在选用相同的软件、建树方法和研究物种的情况下,构建的系统树拓扑结构差异主要是因为线粒体Cyt b和12S rRNA基因进化速率不同产生的。[结论]在分子系统进化研究中,要针对不同的研究物种和研究目的,选择合适的基因构建系统进化树。[Objective] The aim was to prove that the mitochondrial genes of Cyt b and 12S rRNA with different evolutional rates have effects on the topological structures of phylogenetic trees.[Method] The complete sequences of Cyt b and 12S rRNA from 15 species in 12 families of snakes were downloaded and extracted from GenBank,while their molecular phylogenetic trees were constructed by Maximum Likelihood（ML） method with GTR＋I＋G substitute model based on PAUP4.0 software.[Result] With the same software,methods and species,the difference in topological structures of phylogenetic trees was mainly due to different evolutional rates of Cyt b and 12S rRNA genes.[Conclusion] In studies on phylogenetic trees,aimed to different research species and purposes,phylogenetic trees should be constructed by choosing the correct and appropriate genes.

关 键 词：系统进化 蛇 CYTB 12S RRNA 进化速度 

分 类 号：S188[农业科学—农业基础科学]